dc.contributor.author | Brohawn, Stephen G. | |
dc.contributor.author | Schwartz, Thomas | |
dc.date.accessioned | 2012-11-01T20:20:39Z | |
dc.date.available | 2012-11-01T20:20:39Z | |
dc.date.issued | 2009-10 | |
dc.date.submitted | 2009-07 | |
dc.identifier.issn | 1545-9993 | |
dc.identifier.issn | 1545-9985 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/74558 | |
dc.description.abstract | Nuclear pore complexes (NPCs) facilitate all nucleocytoplasmic transport. These massive protein assemblies are modular, with a stable structural scaffold supporting more dynamically attached components. The scaffold is made from multiple copies of the heptameric Y complex and the heteromeric Nic96 complex. We previously showed that members of these core subcomplexes specifically share an ACE1 fold with Sec31 of the COPII vesicle coat, and we proposed a lattice model for the NPC based on this commonality. Here we present the crystal structure of the heterotrimeric 134-kDa complex of Nup84–Nup145C–Sec13 of the Y complex. The heterotypic ACE1 interaction of Nup84 and Nup145C is analogous to the homotypic ACE1 interaction of Sec31 that forms COPII lattice edge elements and is inconsistent with the alternative 'fence-like' NPC model. We construct a molecular model of the Y complex and compare the architectural principles of COPII and NPC lattices. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant GM77537) | en_US |
dc.description.sponsorship | Pew Charitable Trusts (Scholar Award) | en_US |
dc.language.iso | en_US | |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/nsmb.1713 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike 3.0 | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Molecular architecture of the Nup84–Nup145C–Sec13 edge element in the nuclear pore complex lattice | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Brohawn, Stephen G, and Thomas U Schwartz. “Molecular Architecture of the Nup84–Nup145C–Sec13 Edge Element in the Nuclear Pore Complex Lattice.” Nature Structural & Molecular Biology 16.11 (2009): 1173–1177. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.mitauthor | Brohawn, Stephen G. | |
dc.contributor.mitauthor | Schwartz, Thomas | |
dc.relation.journal | Nature Structural & Molecular Biology | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Brohawn, Stephen G; Schwartz, Thomas U | en |
dc.identifier.orcid | https://orcid.org/0000-0001-8012-1512 | |
mit.license | OPEN_ACCESS_POLICY | en_US |
mit.metadata.status | Complete | |