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Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling

dc.contributor.authorUttamapinant, Chayasith
dc.contributor.authorTangpeerachaikul, Anupong
dc.contributor.authorGrecian, Scott
dc.contributor.authorClarke, Scott M.
dc.contributor.authorSingh, Upinder
dc.contributor.authorSlade, Peter
dc.contributor.authorGee, Kyle R.
dc.contributor.authorTing, Alice Y.
dc.date.accessioned2012-11-05T20:25:58Z
dc.date.available2012-11-05T20:25:58Z
dc.date.issued2012-05
dc.date.submitted2011-11
dc.identifier.issn1433-7851
dc.identifier.issn1521-3773
dc.identifier.urihttp://hdl.handle.net/1721.1/74563
dc.description.abstractBring your own copper: Copper-chelating azides undergo much faster click reactions (CuAAC) than nonchelating azides under a variety of biocompatible conditions. This kinetic enhancement allows site-specific protein labeling to be performed on the surface of living cells with only 10–40 μM Cu[superscript I]/Cu[superscript II] (see scheme). Detection sensitivity was also increased for CuAAC detection of alkyne-modified proteins and RNA.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (R01 GM072670)en_US
dc.language.isoen_US
dc.publisherWiley Blackwellen_US
dc.relation.isversionofhttp://dx.doi.org/ 10.1002/anie.201108181en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceProf. Ting via Erja Kajosaloen_US
dc.titleFast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labelingen_US
dc.typeArticleen_US
dc.identifier.citationUttamapinant, Chayasith et al. “Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling.” Angewandte Chemie International Edition 51.24 (2012): 5852–5856.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.contributor.approverTing, Alice Y.
dc.contributor.mitauthorUttamapinant, Chayasith
dc.contributor.mitauthorTangpeerachaikul, Anupong
dc.contributor.mitauthorTing, Alice Y.
dc.relation.journalAngewandte Chemie International Editionen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsUttamapinant, Chayasith; Tangpeerachaikul, Anupong; Grecian, Scott; Clarke, Scott; Singh, Upinder; Slade, Peter; Gee, Kyle R.; Ting, Alice Y.en
dc.identifier.orcidhttps://orcid.org/0000-0002-8277-5226
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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