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An Amyloid Organelle: Solid State NMR Evidence for Cross-Beta Assembly of Gas Vesicles

Author(s)
Bayro, Marvin J.; Daviso, Eugenio; Belenky, Marina; Griffin, Robert Guy; Herzfeld, Judith
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Abstract
Functional amyloids have been identified in a wide range of organisms, taking on a variety of biological roles and being controlled by remarkable mechanisms of directed assembly. Here, we report that amyloid fibrils constitute the ribs of the buoyancy organelles of Anabaena flos-aquae. The walls of these gas-filled vesicles are known to comprise a single protein, GvpA, arranged in a low pitch helix. However, the tertiary and quaternary structures have been elusive. Using solid-state NMR correlation spectroscopy we find detailed evidence for an extended cross-β structure. This amyloid assembly helps to account for the strength and amphiphilic properties of the vesicle wall. Buoyancy organelles thus dramatically extend the scope of known functional amyloids.
Date issued
2012-01
URI
http://hdl.handle.net/1721.1/74602
Department
Massachusetts Institute of Technology. Department of Chemistry; Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology)
Journal
Journal of Biological Chemistry
Publisher
American Society for Biochemistry and Molecular Biology
Citation
Bayro, M. J. et al. “An Amyloid Organelle, Solid-state NMR Evidence for Cross-  Assembly of Gas Vesicles.” Journal of Biological Chemistry 287.5 (2011): 3479–3484. Web.
Version: Author's final manuscript
ISSN
0021-9258
1083-351X

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