An Amyloid Organelle: Solid State NMR Evidence for Cross-Beta Assembly of Gas Vesicles
Author(s)Bayro, Marvin J.; Daviso, Eugenio; Belenky, Marina; Griffin, Robert Guy; Herzfeld, Judith
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Functional amyloids have been identified in a wide range of organisms, taking on a variety of biological roles and being controlled by remarkable mechanisms of directed assembly. Here, we report that amyloid fibrils constitute the ribs of the buoyancy organelles of Anabaena flos-aquae. The walls of these gas-filled vesicles are known to comprise a single protein, GvpA, arranged in a low pitch helix. However, the tertiary and quaternary structures have been elusive. Using solid-state NMR correlation spectroscopy we find detailed evidence for an extended cross-β structure. This amyloid assembly helps to account for the strength and amphiphilic properties of the vesicle wall. Buoyancy organelles thus dramatically extend the scope of known functional amyloids.
DepartmentMassachusetts Institute of Technology. Department of Chemistry; Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology)
Journal of Biological Chemistry
American Society for Biochemistry and Molecular Biology
Bayro, M. J. et al. “An Amyloid Organelle, Solid-state NMR Evidence for Cross- Assembly of Gas Vesicles.” Journal of Biological Chemistry 287.5 (2011): 3479–3484. Web.
Author's final manuscript