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Heterogeneity in mouse spasmolytic polypeptide-expressing metaplasia lineages identifies markers of metaplastic progression

Author(s)
Weis, Victoria G.; Sousa, Josane F.; LaFleur, Bonnie J.; Nam, Ki Taek; Weis, Jared A.; Finke, Paul E.; Ameen, Nadia A.; Fox, James G.; Goldenrign, James R.; ... Show more Show less
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Abstract
Objectives: Spasmolytic polypeptide-expressing metaplasia (SPEM) develops as a preneoplastic lesion in the stomachs of mice and humans after parietal cell loss. To identify the commonalities and differences between phenotypic SPEM lineages, SPEM were studied from three different mouse models of parietal cell loss: with chronic inflammation with Helicobacter felis infection; with acute inflammation with L635 treatment; and without inflammation following DMP-777 treatment. Design: RNA transcripts from laser capture microdissected normal chief cells and SPEM lineages were compared using gene microarray. Alterations in transcripts were validated by quantitative real-time PCR. Clusterin and cystic fibrosis transmembrane conductance regulator (CFTR) were selected for immunohistochemical analysis in all mouse models as well as in human SPEM, intestinal metaplasia and gastric cancer. Results: Transcript expression patterns demonstrated differences among the phenotypic SPEM models. Clusterin expression was significantly upregulated in all three mouse SPEM models as well as in human SPEM. The highest clusterin expression in human gastric cancers correlated with poor survival. Conversely, CFTR expression was upregulated only in SPEM with inflammation in mice. In humans, intestinal metaplasia, but not SPEM, expressed CFTR. Conclusions: While markers such as clusterin are expressed in all phenotypic SPEM lineages, distinct patterns of upregulated genes including CFTR are present in murine metaplasia associated with inflammation, indicative of progression of metaplasia towards a more intestinalised metaplastic phenotype.
Date issued
2012-07
URI
http://hdl.handle.net/1721.1/74663
Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Division of Comparative Medicine
Journal
Gut
Publisher
BMJ Publishing Group
Citation
Weis, V. G. et al. “Heterogeneity in Mouse Spasmolytic Polypeptide-expressing Metaplasia Lineages Identifies Markers of Metaplastic Progression.” Gut (2012): p.1-10. Web. ©2012 BMJ Publishing Group Ltd & British Society of Gastroenterology.
Version: Final published version
ISSN
1468-3288
0017-5749

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