Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system
Author(s)Jones, Christopher T.; Catanese, Maria Teresa; Law, Lok Man J.; Khetani, Salman R.; Syder, Andrew J.; Ploss, Alexander; Oh, Thomas S.; Schoggins, John W.; MacDonald, Margaret R.; Bhatia, Sangeeta N.; Rice, Charles M.; ... Show more Show less
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Hepatitis C virus (HCV), which infects 2–3% of the world population, is a causative agent of chronic hepatitis and the leading indication for liver transplantation1. The ability to propagate HCV in cell culture (HCVcc) is a relatively recent breakthrough and a key tool in the quest for specific antiviral therapeutics. Monitoring HCV infection in culture generally involves bulk population assays, use of genetically modified viruses and/or terminal processing of potentially precious samples. Here we develop a cell-based fluorescent reporter system that allows sensitive distinction of individual HCV-infected cells in live or fixed samples. We demonstrate use of this technology for several previously intractable applications, including live-cell imaging of viral propagation and host response, as well as visualizing infection of primary hepatocyte cultures. Integration of this reporter with modern image-based analysis methods could open new doors for HCV research.
Author Manuscript 2010 August 1
DepartmentHarvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Nature Publishing Group
Jones, Christopher T et al. “Real-time Imaging of Hepatitis C Virus Infection Using a Fluorescent Cell-based Reporter System.” Nature Biotechnology 28.2 (2010): 167–171.
Author's final manuscript