Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production
Author(s)Brigham, Christopher J.; Sinskey, Anthony J.; Song, Yuyang; Matsumoto, Ken’ichiro; Yamada, Miwa; Gohda, Aoi; Taguchi, Seiichi; ... Show more Show less
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The first biosynthetic system for lactate (LA)-based polyesters was previously created in recombinant Escherichia coli (Taguchi et al. 2008). Here, we have begun efforts to upgrade the prototype polymer production system to a practical stage by using metabolically engineered Gram-positive bacterium Corynebacterium glutamicum as an endotoxin-free platform. We designed metabolic pathways in C. glutamicum to generate monomer substrates, lactyl-CoA (LA-CoA), and 3-hydroxybutyryl-CoA (3HB-CoA), for the copolymerization catalyzed by the LA-polymerizing enzyme (LPE). LA-CoA was synthesized by D-lactate dehydrogenase and propionyl-CoA transferase, while 3HB-CoA was supplied by β-ketothiolase (PhaA) and NADPH-dependent acetoacetyl-CoA reductase (PhaB). The functional expression of these enzymes led to a production of P(LA-co-3HB) with high LA fractions (96.8 mol%). The omission of PhaA and PhaB from this pathway led to a further increase in LA fraction up to 99.3 mol%. The newly engineered C. glutamicum potentially serves as a food-grade and biomedically applicable platform for the production of poly(lactic acid)-like polyester.
DepartmentMassachusetts Institute of Technology. Department of Biology
Applied Microbiology and Biotechnology
Song, Yuyang et al. “Engineered Corynebacterium Glutamicum as an Endotoxin-free Platform Strain for Lactate-based Polyester Production.” Applied Microbiology and Biotechnology 93.5 (2011): 1917–1925.
Author's final manuscript