Removing human immunodeficiency virus (HIV) from human blood using immobilized heparin

Author(s)
Nassar, Roger A.; Browne, Edward P.; Chen, Jianzhu; Klibanov, Alexander M.
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Abstract
Heparin covalently attached to a water-insoluble resin suspended in HIV-infected aqueous buffer or whole blood captures the virus; subsequent physical separation of the immobilized heparin reduced the viral titers by over 80 and 50%, respectively. The detoxification concept has been validated by both circulating an HIV-1 solution through a column packed with the heparin–sepharose beads and successively mixing an HIV-1 solution with fresh beads.
Date issued
2011-12
URI
http://hdl.handle.net/1721.1/76226
Department
Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry; Koch Institute for Integrative Cancer Research at MIT
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Citation
Nassar, Roger A. et al. “Removing Human Immunodeficiency Virus (HIV) from Human Blood Using Immobilized Heparin.” Biotechnology Letters 34.5 (2011): 853–856.
Version: Author's final manuscript
ISSN
0141-5492
1573-6776
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