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dc.contributor.authorAnwar, Mekhail
dc.contributor.authorGupta, Piyush
dc.contributor.authorPalaniapan, Raja
dc.contributor.authorMatsudaira, Paul T.
dc.date.accessioned2013-01-23T15:08:19Z
dc.date.available2013-01-23T15:08:19Z
dc.date.issued2012-10
dc.date.submitted2012-04
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1721.1/76339
dc.description.abstractProteins and small molecules are the effectors of physiological action in biological systems and comprehensive methods are needed to analyze their modifications, expression levels and interactions. Systems-scale characterization of the proteome requires thousands of components in high-complexity samples to be isolated and simultaneously probed. While protein microarrays offer a promising approach to probe systems-scale changes in a high-throughput format, they are limited by the need to individually synthesize tens of thousands of proteins. We present an alternative technique, which we call diffusive gel (DiG) stamping, for patterning a microarray using a cellular lysate enabling rapid visualization of dynamic changes in the proteome as well protein interactions. A major advantage of the method described is that it requires no specialized equipment or in-vitro protein synthesis, making it widely accessible to researchers. The method can be integrated with mass spectrometry, allowing for the discovery of novel protein interactions. Here, we describe and characterize the sensitivity and physical features of DiG-Stamping. We demonstrate the biologic utility of DiG-Stamping by (1) identifying the binding partners of a target protein within a cellular lysate and by (2) visualizing the dynamics of proteins with multiple post-translational modifications.en_US
dc.description.sponsorshipWhitaker Foundationen_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pone.0046382en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/en_US
dc.sourcePLoSen_US
dc.titleProtein Array Patterning by Diffusive Gel Stampingen_US
dc.typeArticleen_US
dc.identifier.citationAnwar, Mekhail et al. “Protein Array Patterning by Diffusive Gel Stamping.” Ed. Rizwan Hasan Khan. PLoS ONE 7.10 (2012): e46382. Web.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorMatsudaira, Paul T.
dc.contributor.mitauthorAnwar, Mekhail
dc.contributor.mitauthorGupta, Piyush
dc.contributor.mitauthorPalaniapan, Raja
dc.relation.journalPLoS Oneen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsAnwar, Mekhail; Gupta, Piyush B.; Palaniapan, Raja; Matsudaira, Paulen
dc.identifier.orcidhttps://orcid.org/0000-0002-9703-1780
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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