Exponential Megapriming PCR (EMP) Cloning-Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints
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We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation. Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day. We show that EMP cloning has a higher efficiency than restriction-free (RF) cloning, especially for long inserts above 2.5 kb. EMP further enables simultaneous cloning of multiple inserts.
Date issued
2012-12Department
Massachusetts Institute of Technology. Department of BiologyJournal
PLoS ONE
Publisher
Public Library of Science
Citation
Ulrich, Alexander, Kasper R. Andersen, and Thomas U. Schwartz. “Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid Without Sequence Constraints.” Ed. Mark Isalan. PLoS ONE 7.12 (2012).
Version: Final published version
ISSN
1932-6203