Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy
Author(s)
Luo, Yuan; Zervantonakis, Ioannis; Oh, Se Baek; Kamm, Roger Dale; Barbastathis, George
DownloadKamm_Spectrum resolved.pdf (1.185Mb)
PUBLISHER_POLICY
Publisher Policy
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Terms of use
Metadata
Show full item recordAbstract
A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.
Date issued
2011Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Department of Mechanical EngineeringJournal
Proceedings of SPIE--the International Society for Optical Engineering v.7891
Publisher
SPIE
Citation
Luo, Yuan et al. “Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy.” Proceedings of SPIE--the International Society for Optical Engineering v.7891, 2011. 78910C–78910C–5. © SPIE 2011
Version: Final published version
ISSN
0277-786X