Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy

Author(s)
Luo, Yuan; Zervantonakis, Ioannis; Oh, Se Baek; Kamm, Roger Dale; Barbastathis, George
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Abstract
A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.
Date issued
2011
URI
http://hdl.handle.net/1721.1/78657
Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Department of Mechanical Engineering
Journal
Proceedings of SPIE--the International Society for Optical Engineering v.7891
Publisher
SPIE
Citation
Luo, Yuan et al. “Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy.” Proceedings of SPIE--the International Society for Optical Engineering v.7891, 2011. 78910C–78910C–5. © SPIE 2011
Version: Final published version
ISSN
0277-786X
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