Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy
Author(s)Luo, Yuan; Zervantonakis, Ioannis; Oh, Se Baek; Kamm, Roger Dale; Barbastathis, George
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A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.
DepartmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Department of Mechanical Engineering
Proceedings of SPIE--the International Society for Optical Engineering v.7891
Luo, Yuan et al. “Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy.” Proceedings of SPIE--the International Society for Optical Engineering v.7891, 2011. 78910C–78910C–5. © SPIE 2011
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