Islet Assessment for Transplantation
Author(s)Papas, Klearchos K.; Suszynski, Thomas M.; Colton, Clark K.
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Purpose of review: There is a critical need for meaningful viability and potency assays that characterize islet preparations for release prior to clinical islet cell transplantation (ICT). Development, testing, and validation of such assays have been the subject of intense investigation for the past decade. These efforts are reviewed, highlighting the most recent results while focusing on the most promising assays. Recent Findings: Assays based on membrane integrity do not reflect true viability when applied to either intact islets or dispersed islet cells. Assays requiring disaggregation of intact islets into individual cells for assessment introduce additional problems of cell damage and loss. Assays evaluating mitochondrial function, specifically mitochondrial membrane potential, bioenergetic status, and cellular oxygen consumption rate (OCR), especially when conducted with intact islets, appear most promising in evaluating their quality prior to ICT. Prospective, quantitative assays based on measurements of OCR with intact islets have been developed, validated and their results correlated with transplant outcomes in the diabetic nude mouse bioassay. Conclusion: More sensitive and reliable islet viability and potency tests have been recently developed and tested. Those evaluating mitochondrial function are most promising, correlate with transplant outcomes in mice, and are currently being evaluated in the clinical setting.
Author Manuscript: 2010 December 1.
DepartmentMassachusetts Institute of Technology. Department of Chemical Engineering
Current Opinion in Organ Transplantation
Lippincott Williams & Wilkins
Papas, Klearchos K, Thomas M Suszynski, and Clark K Colton 2009Islet Assessment for Transplantation. Current Opinion in Organ Transplantation 14(6): 674–682.
Author's final manuscript