Self-Assembled Gold Nanoparticle Molecular Probes for Detecting Proteolytic Activity
Author(s)Mu, C. Jenny; LaVan, David A.; Langer, Robert; Zetter, Bruce R.
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Target-activatable fluorogenic probes based on gold nanoparticles (AuNPs) functionalized with self-assembled heterogeneous monolayers of dye-labeled peptides and poly(ethylene glycol) have been developed to visualize proteolytic activity in vivo. A one-step synthesis strategy that allows simple generation of surface-defined AuNP probe libraries is presented as a means of tailoring and evaluating probe characteristics for maximal fluorescence enhancement after protease activation. Optimal AuNP probes targeted to trypsin and urokinase-type plasminogen activator required the incorporation of a dark quencher to achieve 5- to 8-fold signal amplification. These probes exhibited extended circulation time in vivo and high image contrast in a mouse tumor model.
Departmentdelete; Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Chemical Engineering
American Chemical Society
Mu, C. Jenny, David A. LaVan, Robert S. Langer, and Bruce R. Zetter. "Self-Assembled Gold Nanoparticle Molecular Probes for Detecting Proteolytic Activity In Vivo." ACS Nano 4, no. 3 (March 23, 2010): 1511-1520.
Author's final manuscript