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dc.contributor.authorSong, Qing
dc.contributor.authorHan, Qing
dc.contributor.authorBradshaw, Elizabeth M.
dc.contributor.authorKent, Sally C.
dc.contributor.authorRaddassi, Khadir
dc.contributor.authorNilsson, Bjorn
dc.contributor.authorNepom, Gerald T.
dc.contributor.authorHafler, David A.
dc.contributor.authorLove, J. Christopher
dc.date.accessioned2013-06-26T15:09:09Z
dc.date.available2013-06-26T15:09:09Z
dc.date.issued2009-12
dc.date.submitted2009-10
dc.identifier.issn0003-2700
dc.identifier.issn1520-6882
dc.identifier.urihttp://hdl.handle.net/1721.1/79369
dc.description.abstractThe frequencies of antigen-specific CD4+ T cells in samples of human tissue have been difficult to determine accurately ex vivo, particularly for autoimmune diseases such as multiple sclerosis or type 1 diabetes. Conventional approaches involve the expansion of primary T cells in vitro to increase the numbers of cells, and a subsequent assessment of the frequencies of antigen-specific T cells in the expanded population by limiting dilution or by using fluorescently labeled tetramers of peptide-loaded major histocompatibility complex (MHC) receptors. Here we describe an alternative approach that uses arrays of subnanoliter wells coated with recombinant peptide-loaded MHC class II monomers to isolate and stimulate individual CD4+ T cells in an antigen-specific manner. In these experiments, activation was monitored using microengraving to capture two cytokines (IFNγ and IL-17) released from single cells. This new method should enable direct enumeration of antigen-specific CD4+ T cells ex vivo from clinical samples.en_US
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases (U.S.) (Award Number 5U19AI050864-07)en_US
dc.description.sponsorshipJuvenile Diabetes Research Foundation Internationalen_US
dc.description.sponsorshipMassachusetts Institute of Technology (Texaco- Mangelsdorf Career Development Professor)en_US
dc.language.isoen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/ac9024363en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleOn-Chip Activation and Subsequent Detection of Individual Antigen-Specific T Cellsen_US
dc.typeArticleen_US
dc.identifier.citationSong, Qing, Qing Han, Elizabeth M. Bradshaw, Sally C. Kent, Khadir Raddassi, Björn Nilsson, Gerald T. Nepom, David A. Hafler, and J. Christopher Love. On-Chip Activation and Subsequent Detection of Individual Antigen-Specific T Cells. Analytical Chemistry 82, no. 2 (January 15, 2010): 473-477.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.mitauthorSong, Qingen_US
dc.contributor.mitauthorHan, Qingen_US
dc.contributor.mitauthorLove, Christopher J.en_US
dc.relation.journalAnalytical Chemistryen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsSong, Qing; Han, Qing; Bradshaw, Elizabeth M.; Kent, Sally C.; Raddassi, Khadir; Nilsson, Björn; Nepom, Gerald T.; Hafler, David A.; Love, J. Christopheren_US
dc.identifier.orcidhttps://orcid.org/0000-0002-7989-2376
dspace.mitauthor.errortrue
mit.licensePUBLISHER_POLICYen_US


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