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dc.contributor.authorWen, Jian
dc.contributor.authorAlbrecht, Jacob W.
dc.contributor.authorJensen, Klavs F.
dc.date.accessioned2013-06-26T16:01:32Z
dc.date.available2013-06-26T16:01:32Z
dc.date.issued2010-04
dc.date.issued2010-04
dc.identifier.issn01730835en_US
dc.identifier.issn15222683en_US
dc.identifier.urihttp://hdl.handle.net/1721.1/79374
dc.description.abstractA preparative scale free-flow IEF device is developed and characterized with the aim of addressing needs of molecular biologists working with protein samples on the milligrams and milliliters scale. A triangular-shape separation channel facilitates the establishment of the pH gradient with a corresponding increase in separation efficiency and decrease in focusing time compared with that in a regular rectangular channel. Functionalized, ion-permeable poly(acrylamide) gel membranes are sandwiched between PDMS and glass layers to both isolate the electrode buffers from the central separation channel and also to selectively adjust the voltage efficiency across the separation channel to achieve high electric field separation. The 50×70 mm device is fabricated by soft lithography and has 24 outlets evenly spaced across a pH gradient between pH 4 and 10. This preparative free-flow IEF system is investigated and optimized for both aqueous and denaturing conditions with respect to the electric field and potential efficiency and with consideration of Joule-heating removal. Energy distribution across the functionalized polyacrylamide gel is investigated and controlled to adjust the potential efficiency between 15 and 80% across the triangular separation channel. The device is able to achieve constant electric fields high as 370±20 V/cm through the entire triangular channel given the separation voltage of 1800 V, enabling separation of five fluorescent pI markers as a demonstration example.
dc.description.sponsorshipUnited States. Army Research Office (Grant Number: W911NF-07-D-0004)
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant Number: GM68762)
dc.language.isoen_US
dc.publisherJohn Wiley & Sons, Inc.
dc.relation.isversionofhttp://dx.doi.org/10.1002/elps.200900577en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourcePMCen_US
dc.titleMicrofluidic preparative free-flow isoelectric focusing in a triangular channel: System development and characterizationen_US
dc.typeArticleen_US
dc.identifier.citationWen, Jian, Jacob W. Albrecht, and Klavs F. Jensen. Microfluidic Preparative Free-flow Isoelectric Focusing in a Triangular Channel: System Development and Characterization. Electrophoresis 31, no. 10 (May 23, 2010): 1606-1614.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.mitauthorJensen, Klavs F.
dc.relation.journalELECTROPHORESISen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsWen, Jian; Albrecht, Jacob W.; Jensen, Klavs F.en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7192-580X
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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