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dc.contributor.authorYamanaka, Yvonne Joy
dc.contributor.authorSzeto, Gregory Lee
dc.contributor.authorGierahn, Todd Michael
dc.contributor.authorForcier, Talitha L.
dc.contributor.authorBenedict, Kelly
dc.contributor.authorBrefo, Mavis S.
dc.contributor.authorLauffenburger, Douglas A.
dc.contributor.authorIrvine, Darrell J.
dc.contributor.authorLove, J. Christopher
dc.date.accessioned2013-07-26T15:15:15Z
dc.date.available2013-07-26T15:15:15Z
dc.date.issued2012-12
dc.identifier.issn0003-2700
dc.identifier.issn1520-6882
dc.identifier.urihttp://hdl.handle.net/1721.1/79706
dc.description.abstractWe present a method that uses fluorescent cellular barcodes to increase the number of unique samples that can be analyzed simultaneously by microengraving, a nanowell array-based technique for quantifying the secretory responses of thousands of single cells in parallel. Using n different fluorescent dyes to generate 2n unique cellular barcodes, we achieved a 2n-fold reduction in the number of arrays and quantity of reagents required per sample. The utility of this approach was demonstrated in three applications of interest in clinical and experimental immunology. Using barcoded human peripheral blood mononuclear cells and T cells, we constructed dose–response curves, profiled the secretory behavior of cells treated with mechanistically distinct stimuli, and tracked the secretory behaviors of different lineages of CD4+ T helper cells. In addition to increasing the number of samples analyzed by generating secretory profiles of single cells from multiple populations in a time- and reagent-efficient manner, we expect that cellular barcoding in combination with microengraving will facilitate unique experimental opportunities for quantitatively analyzing interactions among heterogeneous cells isolated in small groups (2–5 cells).en_US
dc.description.sponsorshipHoward Hughes Medical Institute (Investigator)en_US
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases (U.S.) (Grant 1R56AI104274)en_US
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases (U.S.) (Grant 1U19AI089992)en_US
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases (U.S.) (Grant 5U01AI068618)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Fellowship)en_US
dc.description.sponsorshipMassachusetts Institute of Technology (Collamore-Rogers Fellowship)en_US
dc.description.sponsorshipRagon Institute of MGH, MIT and Harvarden_US
dc.description.sponsorshipW. M. Keck Foundationen_US
dc.language.isoen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/ac302264qen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceProf. Love via Erja Kajosaloen_US
dc.titleCellular Barcodes for Efficiently Profiling Single-Cell Secretory Responses by Microengravingen_US
dc.typeArticleen_US
dc.identifier.citationYamanaka, Yvonne J., Gregory L. Szeto, Todd M. Gierahn, et al. 2012 Cellular Barcodes for Efficiently Profiling Single-Cell Secretory Responses by Microengraving. Analytical Chemistry 84(24): 10531–10536.en_US
dc.contributor.departmentDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Materials Science and Engineeringen_US
dc.contributor.departmentRagon Institute of MGH, MIT and Harvarden_US
dc.contributor.mitauthorLove, J. Christopheren_US
dc.contributor.mitauthorYamanaka, Yvonne Joyen_US
dc.contributor.mitauthorSzeto, Gregory Leeen_US
dc.contributor.mitauthorGierahn, Todd Michaelen_US
dc.contributor.mitauthorForcier, Talitha L.en_US
dc.contributor.mitauthorBenedict, Kellyen_US
dc.contributor.mitauthorBrefo, Mavis S.en_US
dc.contributor.mitauthorLauffenburger, Douglas A.en_US
dc.contributor.mitauthorIrvine, Darrell J.en_US
dc.relation.journalAnalytical Chemistryen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsYamanaka, Yvonne J.; Szeto, Gregory L.; Gierahn, Todd M.; Forcier, Talitha L.; Benedict, Kelly F.; Brefo, Mavis S.N.; Lauffenburger, Douglas A.; Irvine, Darrell J.; Love, J. Christopheren_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7604-1333
dc.identifier.orcidhttps://orcid.org/0000-0003-0921-3144
mit.licensePUBLISHER_POLICYen_US


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