Regulation of thymocyte positive selection and motility by GIT2
Author(s)Phee, Hyewon; Dzhagalov, Ivan; Mollenauer, Marianne; Wang, Yana; Irvine, Darrell J.; Robey, Ellen; Weiss, Arthur; ... Show more Show less
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Thymocytes are highly motile cells that migrate under the influence of chemokines in distinct thymic compartments as they mature. The motility of thymocytes is tightly regulated; however, the molecular mechanisms that control thymocyte motility are not well understood. Here we report that G protein–coupled receptor kinase-interactor 2 (GIT2) was required for efficient positive selection. Notably, Git2−/− double-positive thymocytes showed greater activation of the small GTPase Rac, actin polymerization and migration toward the chemokines CXCL12 (SDF-1) and CCL25 in vitro. By two-photon laser-scanning microscopy, we found that the scanning activity of Git2−/− thymocytes was compromised in the thymic cortex, which suggests GIT2 has a key role in regulating the chemokine-mediated motility of double-positive thymocytes.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Materials Science and Engineering
Nature Publishing Group
Phee, Hyewon, Ivan Dzhagalov, Marianne Mollenauer, Yana Wang, Darrell J Irvine, Ellen Robey, and Arthur Weiss. “Regulation of thymocyte positive selection and motility by GIT2.” Nature Immunology 11, no. 6 (May 2, 2010): 503-511.
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