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dc.contributor.authorZhang, Feng
dc.contributor.authorBikard, David
dc.contributor.authorJiang, Wenyan
dc.contributor.authorSamai, Poulami
dc.contributor.authorHochschild, Ann
dc.contributor.authorMarraffini, Luciano A.
dc.date.accessioned2013-07-31T17:56:54Z
dc.date.available2013-07-31T17:56:54Z
dc.date.issued2013-06
dc.date.submitted2013-05
dc.identifier.issn0305-1048
dc.identifier.issn1362-4962
dc.identifier.urihttp://hdl.handle.net/1721.1/79745
dc.description.abstractThe ability to artificially control transcription is essential both to the study of gene function and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA-guided double-stranded DNA nuclease that participates in the CRISPR-Cas immune defense against prokaryotic viruses. We describe the use of a Cas9 nuclease mutant that retains DNA-binding activity and can be engineered as a programmable transcription repressor by preventing the binding of the RNA polymerase (RNAP) to promoter sequences or as a transcription terminator by blocking the running RNAP. In addition, a fusion between the omega subunit of the RNAP and a Cas9 nuclease mutant directed to bind upstream promoter regions can achieve programmable transcription activation. The simple and efficient modulation of gene expression achieved by this technology is a useful asset for the study of gene networks and for the development of synthetic biology and biotechnological applications.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Pioneer Award DP1MH100706)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Transformative Research Award)en_US
dc.description.sponsorshipW. M. Keck Foundationen_US
dc.description.sponsorshipMcKnight Foundationen_US
dc.description.sponsorshipBill & Melinda Gates Foundationen_US
dc.description.sponsorshipDamon Runyon Cancer Research Foundationen_US
dc.description.sponsorshipKinship Foundation. Searle Scholars Programen_US
dc.description.sponsorshipEsther A. & Joseph Klingenstein Fund, Inc.en_US
dc.description.sponsorshipSimons Foundationen_US
dc.language.isoen_US
dc.publisherOxford University Pressen_US
dc.relation.isversionofhttp://dx.doi.org/10.1093/nar/gkt520en_US
dc.rightsCreative Commons Attribution Non-Commercialen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0en_US
dc.sourceOxford University Pressen_US
dc.titleProgrammable repression and activation of bacterial gene expression using an engineered CRISPR-Cas systemen_US
dc.typeArticleen_US
dc.identifier.citationBikard, D. et al. “Programmable Repression and Activation of Bacterial Gene Expression Using an Engineered CRISPR-Cas System.” Nucleic Acids Research (2013).en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.contributor.mitauthorZhang, Fengen_US
dc.relation.journalNucleic Acids Researchen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsBikard, D.; Jiang, W.; Samai, P.; Hochschild, A.; Zhang, F.; Marraffini, L. A.en_US
dc.identifier.orcidhttps://orcid.org/0000-0003-2782-2509
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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