dc.contributor.author | Zhang, Feng | |
dc.contributor.author | Bikard, David | |
dc.contributor.author | Jiang, Wenyan | |
dc.contributor.author | Samai, Poulami | |
dc.contributor.author | Hochschild, Ann | |
dc.contributor.author | Marraffini, Luciano A. | |
dc.date.accessioned | 2013-07-31T17:56:54Z | |
dc.date.available | 2013-07-31T17:56:54Z | |
dc.date.issued | 2013-06 | |
dc.date.submitted | 2013-05 | |
dc.identifier.issn | 0305-1048 | |
dc.identifier.issn | 1362-4962 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/79745 | |
dc.description.abstract | The ability to artificially control transcription is essential both to the study of gene function and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA-guided double-stranded DNA nuclease that participates in the CRISPR-Cas immune defense against prokaryotic viruses. We describe the use of a Cas9 nuclease mutant that retains DNA-binding activity and can be engineered as a programmable transcription repressor by preventing the binding of the RNA polymerase (RNAP) to promoter sequences or as a transcription terminator by blocking the running RNAP. In addition, a fusion between the omega subunit of the RNAP and a Cas9 nuclease mutant directed to bind upstream promoter regions can achieve programmable transcription activation. The simple and efficient modulation of gene expression achieved by this technology is a useful asset for the study of gene networks and for the development of synthetic biology and biotechnological applications. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Pioneer Award DP1MH100706) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Transformative Research Award) | en_US |
dc.description.sponsorship | W. M. Keck Foundation | en_US |
dc.description.sponsorship | McKnight Foundation | en_US |
dc.description.sponsorship | Bill & Melinda Gates Foundation | en_US |
dc.description.sponsorship | Damon Runyon Cancer Research Foundation | en_US |
dc.description.sponsorship | Kinship Foundation. Searle Scholars Program | en_US |
dc.description.sponsorship | Esther A. & Joseph Klingenstein Fund, Inc. | en_US |
dc.description.sponsorship | Simons Foundation | en_US |
dc.language.iso | en_US | |
dc.publisher | Oxford University Press | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1093/nar/gkt520 | en_US |
dc.rights | Creative Commons Attribution Non-Commercial | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0 | en_US |
dc.source | Oxford University Press | en_US |
dc.title | Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Bikard, D. et al. “Programmable Repression and Activation of Bacterial Gene Expression Using an Engineered CRISPR-Cas System.” Nucleic Acids Research (2013). | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences | en_US |
dc.contributor.department | McGovern Institute for Brain Research at MIT | en_US |
dc.contributor.mitauthor | Zhang, Feng | en_US |
dc.relation.journal | Nucleic Acids Research | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Bikard, D.; Jiang, W.; Samai, P.; Hochschild, A.; Zhang, F.; Marraffini, L. A. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-2782-2509 | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |