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dc.contributor.authorCarette, Jan E.
dc.contributor.authorBrummelkamp, Thijn R.
dc.contributor.authorLee, Clarissa Ci
dc.contributor.authorPloegh, Hidde
dc.date.accessioned2013-08-14T14:05:34Z
dc.date.available2013-08-14T14:05:34Z
dc.date.issued2013-07
dc.date.submitted2013-03
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1721.1/79854
dc.description.abstractThe development of forward genetic screens in human haploid cells has the potential to transform our understanding of the genetic basis of cellular processes unique to man. So far, this approach has been limited mostly to the identification of genes that mediate cell death in response to a lethal agent, likely due to the ease with which this phenotype can be observed. Here, we perform the first reporter screen in the near-haploid KBM7 cell line to identify constitutive inhibitors of NF-κB. CYLD was the only currently known negative regulator of NF-κB to be identified, thus uniquely distinguishing this gene. Also identified were three genes with no previous known connection to NF-κB. Our results demonstrate that reporter screens in haploid human cells can be applied to investigate the many complex signaling pathways that converge upon transcription factors.en_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pone.0070339en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/en_US
dc.sourcePLoSen_US
dc.titleA Reporter Screen in a Human Haploid Cell Line Identifies CYLD as a Constitutive Inhibitor of NF-κBen_US
dc.typeArticleen_US
dc.identifier.citationLee, Clarissa C., Jan E. Carette, Thijn R. Brummelkamp, and Hidde L. Ploegh. “A Reporter Screen in a Human Haploid Cell Line Identifies CYLD as a Constitutive Inhibitor of NF-κB.” Edited by Alexander Poltorak. PLoS ONE 8, no. 7 (July 8, 2013): e70339.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorLee, Clarissa Cien_US
dc.contributor.mitauthorPloegh, Hiddeen_US
dc.relation.journalPLoS ONEen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLee, Clarissa C.; Carette, Jan E.; Brummelkamp, Thijn R.; Ploegh, Hidde L.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-1090-6071
mit.licensePUBLISHER_CCen_US


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