Divergent transcription of long noncoding RNA/mRNA gene pairs in embryonic stem cells

Author(s)
Almada, Albert Ernesto; Lin, Charles; Sharp, Phillip A.; Young, Richard A.; Sigova, Alla A.; ... Show more
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Abstract
Many long noncoding RNA (lncRNA) species have been identified in mammalian cells, but the genomic origin and regulation of these molecules in individual cell types is poorly understood. We have generated catalogs of lncRNA species expressed in human and murine embryonic stem cells and mapped their genomic origin. A surprisingly large fraction of these transcripts (>60%) originate from divergent transcription at promoters of active protein-coding genes. The divergently transcribed lncRNA/mRNA gene pairs exhibit coordinated changes in transcription when embryonic stem cells are differentiated into endoderm. Our results reveal that transcription of most lncRNA genes is coordinated with transcription of protein-coding genes.
Date issued
2013-02
URI
http://hdl.handle.net/1721.1/80396
Department
Massachusetts Institute of Technology. Department of Biology; Koch Institute for Integrative Cancer Research at MIT
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Sigova, A. A., A. C. Mullen, B. Molinie, S. Gupta, D. A. Orlando, M. G. Guenther, A. E. Almada, et al. “Divergent transcription of long noncoding RNA/mRNA gene pairs in embryonic stem cells.” Proceedings of the National Academy of Sciences 110, no. 8 (February 19, 2013): 2876-2881.
Version: Final published version
ISSN
0027-8424
1091-6490
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