Long noncoding RNAs regulate adipogenesis
Author(s)Sun, Lei; Goff, Loyal; Alexander, Ryan K.; Lo, Kinyui Alice; Yuan, Bingbing B.; Kellis, Manolis; Lodish, Harvey F.; Trapnell, Cole; Hacisuleyman, Ezgi; Sauvageau, Martin; Tazon-Vega, Barbara; Kelley, David R.; Hendrickson, David G.; Rinn, John L.; ... Show more Show less
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The prevalence of obesity has led to a surge of interest in understanding the detailed mechanisms underlying adipocyte development. Many protein-coding genes, mRNAs, and microRNAs have been implicated in adipocyte development, but the global expression patterns and functional contributions of long noncoding RNA (lncRNA) during adipogenesis have not been explored. Here we profiled the transcriptome of primary brown and white adipocytes, preadipocytes, and cultured adipocytes and identified 175 lncRNAs that are specifically regulated during adipogenesis. Many lncRNAs are adipose-enriched, strongly induced during adipogenesis, and bound at their promoters by key transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (CEBPα). RNAi-mediated loss of function screens identified functional lncRNAs with varying impact on adipogenesis. Collectively, we have identified numerous lncRNAs that are functionally required for proper adipogenesis.
DepartmentMassachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Whitehead Institute for Biomedical Research
Proceedings of the National Academy of Sciences
National Academy of Sciences (U.S.)
Sun, L., L. A. Goff, C. Trapnell, R. Alexander, K. A. Lo, E. Hacisuleyman, M. Sauvageau, et al. “Long noncoding RNAs regulate adipogenesis.” Proceedings of the National Academy of Sciences 110, no. 9 (February 26, 2013): 3387-3392.
Final published version