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Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained via SCNT

Author(s)
Suh, Heikyung; Jaenisch, Rudolf; Ploegh, Hidde; Kirak, Oktay; Frickel, Eva-Maria; Grotenbreg, Gijsbert M.; ... Show more Show less
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Alternative title
Transnuclear Mice with Predefined T Cell Receptor Specificities Against Toxoplasma gondii Obtained via SCNT
Terms of use
Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/
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Abstract
Mice that are transgenic for rearranged antigen-specific T cell receptors (TCRs) are essential tools to study T cell development and function. Such TCRs are usually isolated from the relevant T cells after long-term culture, often after repeated antigen stimulation, which unavoidably skews the T cell population used. Random genomic integration of the TCR α and β chain and expression from nonendogenous promoters represent additional drawbacks of transgenics. Using epigenetic reprogramming via somatic cell nuclear transfer, we demonstrated that T cells with predefined specificities against Toxoplasma gondii can be used to generate mouse models that express the TCR from their endogenous loci, without experimentally introduced genetic modification. The relative ease and speed with which such transnuclear models can be obtained holds promise for the construction of other disease models.
Date issued
2010-04
URI
http://hdl.handle.net/1721.1/81211
Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology; Whitehead Institute for Biomedical Research
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Kirak, O., E.-M. Frickel, G. M. Grotenbreg, H. Suh, R. Jaenisch, and H. L. Ploegh. “Transnuclear Mice with Predefined T Cell Receptor Specificities Against Toxoplasma gondii Obtained via SCNT.” Science 328, no. 5975 (April 8, 2010): 243-248.
Version: Author's final manuscript
ISSN
0036-8075
1095-9203

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