The mTOR-Regulated Phosphoproteome Reveals a Mechanism of mTORC1-Mediated Inhibition of Growth Factor Signaling
Author(s)
Rameseder, J.; Zhang, Y.; Ottina, Kathleen; Lim, D.; Choi, Y.; Gray, Nathanael S.; Yaffe, M. B.; Marto, Jarrod A.; Hsu, Peggy P.; Kang, Seong A.; Peterson, Timothy R.; Sabatini, David M.; ... Show more Show less
DownloadSabatini_The mTOR.pdf (1.670Mb)
OPEN_ACCESS_POLICY
Open Access Policy
Creative Commons Attribution-Noncommercial-Share Alike
Terms of use
Metadata
Show full item recordAbstract
The mammalian target of rapamycin (mTOR) protein kinase is a master growth promoter that nucleates two complexes, mTORC1 and mTORC2. Despite the diverse processes controlled by mTOR, few substrates are known. We defined the mTOR-regulated phosphoproteome by quantitative mass spectrometry and characterized the primary sequence motif specificity of mTOR using positional scanning peptide libraries. We found that the phosphorylation response to insulin is largely mTOR dependent and that mTOR exhibits a unique preference for proline, hydrophobic, and aromatic residues at the +1 position. The adaptor protein Grb10 was identified as an mTORC1 substrate that mediates the inhibition of phosphoinositide 3-kinase typical of cells lacking tuberous sclerosis complex 2 (TSC2), a tumor suppressor and negative regulator of mTORC1. Our work clarifies how mTORC1 inhibits growth factor signaling and opens new areas of investigation in mTOR biology.
Description
September 21 Author Manuscript
Date issued
2011-04Department
Massachusetts Institute of Technology. Department of BiologyJournal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Hsu, P. P., S. A. Kang, J. Rameseder, Y. Zhang, K. A. Ottina, D. Lim, T. R. Peterson, et al. “The mTOR-Regulated Phosphoproteome Reveals a Mechanism of mTORC1-Mediated Inhibition of Growth Factor Signaling.” Science 332, no. 6035 (June 9, 2011): 1317-1322.
Version: Author's final manuscript
ISSN
0036-8075
1095-9203