Proton Assisted Recoupling at High Spinning Frequencies
Author(s)Struppe, Jochem; Maas, Werner; Griffin, Robert Guy; Lewandowski, Jozef R.; Eddy, Matthew Thomas; Paepe, Gael De; ... Show more Show less
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We demonstrate the successful application of [superscript 13]C−[superscript 13]C proton assisted recoupling (PAR) on [U−[superscript 13]C,[superscript 15]N] N-f-MLF-OH and [U−13C,15N] protein GB1 at high magic angle spinning (MAS) frequencies (ω[subscript r]/2π = 65 kHz). Specifically, by combining PAR mixing with low power heteronuclear decoupling (ω[subscript 1H]/2π~16 kHz) and high spinning frequencies, we obtain high resolution 2D spectra displaying long-range [superscript 13]C−[superscript 13]C contacts from which distance estimates can be extracted. These experiments therefore demonstrate the possibility of performing high resolution structural studies in the limit of high spinning frequency and low power [superscript 1]H decoupling, a regime which optimizes the resolution of protein samples and preserves their integrity.
DepartmentMassachusetts Institute of Technology. Department of Chemistry; Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology)
Journal of Physical Chemistry B
American Chemical Society (ACS)
Lewandowski, Jozef R., Gael De Paepe, Matthew T. Eddy, Jochem Struppe, Werner Maas, and Robert G. Griffin. “Proton Assisted Recoupling at High Spinning Frequencies.” The Journal of Physical Chemistry B 113, no. 27 (July 9, 2009): 9062-9069.
Author's final manuscript