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dc.contributor.authorDrinnenberg, Ines A.
dc.contributor.authorWeinberg, David E.
dc.contributor.authorXie, Kathleen Dongqin
dc.contributor.authorMower, Jeffrey P.
dc.contributor.authorWolfe, Kenneth H.
dc.contributor.authorFink, Gerald R.
dc.contributor.authorBartel, David
dc.date.accessioned2013-12-19T20:04:25Z
dc.date.available2013-12-19T20:04:25Z
dc.date.issued2009-09
dc.date.submitted2009-05
dc.identifier.issn0036-8075
dc.identifier.issn1095-9203
dc.identifier.urihttp://hdl.handle.net/1721.1/83084
dc.description.abstractRNA interference (RNAi), a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast Saccharomyces cerevisiae. Here, we show that RNAi is present in other budding yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate small interfering RNAs, which mostly correspond to transposable elements and Y′ subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess Y′ messenger RNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a previously unknown class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi.en_US
dc.language.isoen_US
dc.publisherAmerican Association for the Advancement of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1126/science.1176945en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourcePMCen_US
dc.titleRNAi in Budding Yeasten_US
dc.typeArticleen_US
dc.identifier.citationDrinnenberg, I. A., D. E. Weinberg, K. T. Xie, J. P. Mower, K. H. Wolfe, G. R. Fink, and D. P. Bartel. “RNAi in Budding Yeast.” Science 326, no. 5952 (October 22, 2009): 544-550.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorDrinnenberg, Ines A.en_US
dc.contributor.mitauthorWeinberg, David E.en_US
dc.contributor.mitauthorXie, Kathleen Dongqinen_US
dc.contributor.mitauthorFink, Gerald R.en_US
dc.contributor.mitauthorBartel, Daviden_US
dc.relation.journalScienceen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsDrinnenberg, I. A.; Weinberg, D. E.; Xie, K. T.; Mower, J. P.; Wolfe, K. H.; Fink, G. R.; Bartel, D. P.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-3872-2856
dc.identifier.orcidhttps://orcid.org/0000-0003-3704-2899
mit.licenseOPEN_ACCESS_POLICYen_US


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