Critical Role of Ena/VASP Proteins for Filopodia Formation in Neurons and in Function Downstream of Netrin-1
Author(s)Lebrand, Cecile; Lanier, Lorene M.; Krause, Matthias; Svitkina, Tatyana M.; Borisy, Gary G.; Dent, Erik W.; Strasser, Geraldine A.; Gertler, Frank; ... Show more Show less
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Ena/VASP proteins play important roles in axon outgrowth and guidance. Ena/VASP activity regulates the assembly and geometry of actin networks within fibroblast lamellipodia. In growth cones, Ena/VASP proteins are concentrated at filopodia tips, yet their role in growth cone responses to guidance signals has not been established. We found that Ena/VASP proteins play a pivotal role in formation and elongation of filopodia along neurite shafts and growth cone. Netrin-1-induced filopodia formation was dependent upon Ena/VASP function and directly correlated with Ena/VASP phosphorylation at a regulatory PKA site. Accordingly, Ena/VASP function was required for filopodial formation from the growth cone in response to global PKA activation. We propose that Ena/VASP proteins control filopodial dynamics in neurons by remodeling the actin network in response to guidance cues.
DepartmentMassachusetts Institute of Technology. Department of Biology
Lebrand, Cecile, Erik W Dent, Geraldine A Strasser, Lorene M Lanier, Matthias Krause, Tatyana M Svitkina, Gary G Borisy, and Frank B Gertler. “Critical Role of Ena/VASP Proteins for Filopodia Formation in Neurons and in Function Downstream of Netrin-1.” Neuron 42, no. 1 (April 2004): 37-49. Copyright © 2004 Cell Press
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