dc.contributor.author | Lin, Daniel Chi-Hong | |
dc.contributor.author | Grossman, Alan Davis | |
dc.date.accessioned | 2014-01-10T15:06:36Z | |
dc.date.available | 2014-01-10T15:06:36Z | |
dc.date.issued | 1998-03 | |
dc.date.submitted | 1998-02 | |
dc.identifier.issn | 00928674 | |
dc.identifier.issn | 1097-4172 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/83851 | |
dc.description.abstract | We have identified a DNA site involved in chromosome partitioning in B. subtilis. This site was identified in vivo as the binding site for the chromosome partitioning protein Spo0J, a member of the ParB family of partitioning proteins. Spo0J is a site-specific DNA-binding protein that recognizes a 16 bp sequence found in spo0J. Allowing two mismatches, this sequence occurs ten times in the entire B. subtilis chromosome, all in the origin-proximal ~20%. Eight of the ten sequences are bound to Spo0J in vivo. The presence of a site on an otherwise unstable plasmid stabilized the plasmid in a Spo0J-dependent manner, demonstrating that this site, called parS, can function as a partitioning site. This site and Spo0J are conserved in a wide range of bacterial species. | en_US |
dc.description.sponsorship | National Science Foundation (U.S.) (Predoctoral Training Grant) | en_US |
dc.description.sponsorship | Lucille P. Markey Charitable Trust | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Public Health Services Grant GM41934) | en_US |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1016/S0092-8674(00)81135-6 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | Elsevier Open Archive | en_US |
dc.title | Identification and Characterization of a Bacterial Chromosome Partitioning Site | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Lin, Daniel Chi-Hong, and Alan D Grossman. “Identification and Characterization of a Bacterial Chromosome Partitioning Site.” Cell 92, no. 5 (March 1998): 675-685. Copyright © 1998 Cell Press | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.mitauthor | Lin, Daniel Chi-Hong | en_US |
dc.contributor.mitauthor | Grossman, Alan D. | en_US |
dc.relation.journal | Cell | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Lin, Daniel Chi-Hong; Grossman, Alan D | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-8235-7227 | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |