Bipolar Localization of the Replication Origin Regions of Chromosomes in Vegetative and Sporulating Cells of B. subtilis
Author(s)
Webb, Chris D; Teleman, Aurelio; Gordon, Scott; Straight, Aaron; Belmont, Andrew; Lin, Daniel Chi-Hong; Wright, Andrew; Losick, Richard; Grossman, Alan Davis; ... Show more Show less
DownloadGrossman_Bipolar localization.pdf (509.2Kb)
PUBLISHER_POLICY
Publisher Policy
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Terms of use
Metadata
Show full item recordAbstract
To investigate chromosome segregation in B. subtilis, we introduced tandem copies of the lactose operon operator into the chromosome near the replication origin or terminus. We then visualized the position of the operator cassettes with green fluorescent protein fused to the LacI repressor. In sporulating bacteria, which undergo asymmetric cell division, origins localized near each pole of the cell whereas termini were restricted to the middle. In growing cells, which undergo binary fission, origins were observed at various positions but preferentially toward the poles early in the cell cycle. In contrast, termini showed little preference for the poles. These results indicate the existence of a mitotic-like apparatus that is responsible for moving the origin regions of newly formed chromosomes toward opposite ends of the cell.
Date issued
1997-03Department
Massachusetts Institute of Technology. Department of BiologyJournal
Cell
Publisher
Elsevier
Citation
Webb, Chris D, Aurelio Teleman, Scott Gordon, Aaron Straight, Andrew Belmont, Daniel Chi-Hong Lin, Alan D Grossman, Andrew Wright, and Richard Losick. “Bipolar Localization of the Replication Origin Regions of Chromosomes in Vegetative and Sporulating Cells of B. subtilis.” Cell 88, no. 5 (March 1997): 667-674. Copyright © 1997 Cell Press
Version: Final published version
ISSN
00928674
1097-4172