Visualization of Mismatch Repair in Bacterial Cells

Author(s)
Smith, Bradley T.; Walker, Graham C.; Grossman, Alan Davis
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Abstract
We determined the localizations of mismatch repair proteins in living Bacillus subtilis cells. MutS-GFP colocalized with the chromosome in all cells and formed foci in a subset of cells. MutL-GFP formed foci in a subset of cells, and its localization was MutS dependent. The introduction of mismatches by growth in 2-aminopurine caused a replication-dependent increase in the number of cells with MutS and MutL foci. Approximately half of the MutS foci colocalized with DNA polymerase foci. We conclude that MutS is associated with the entire chromosome, poised to detect mismatches. After detection, it appears that mismatch repair foci assemble at mismatches as they emerge from the DNA polymerase and are then carried away from the replisome by continuing replication.
Date issued
2001-12
URI
http://hdl.handle.net/1721.1/83855
Department
Massachusetts Institute of Technology. Department of Biology
Journal
Molecular Cell
Publisher
Elsevier
Citation
Smith, Bradley T, Alan D Grossman, and Graham C Walker. “Visualization of Mismatch Repair in Bacterial Cells.” Molecular Cell 8.6 (2001): 1197–1206. Copyright © 2001 Cell Press
Version: Final published version
ISSN
10972765
1097-4164
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