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dc.contributor.authorLazazzera, Beth A.
dc.contributor.authorSolomon, Jonathan M.
dc.contributor.authorGrossman, Alan Davis
dc.date.accessioned2014-01-10T20:56:53Z
dc.date.available2014-01-10T20:56:53Z
dc.date.issued1997-06
dc.date.submitted1997-05
dc.identifier.issn00928674
dc.identifier.issn1097-4172
dc.identifier.urihttp://hdl.handle.net/1721.1/83874
dc.description.abstractCompetence development and sporulation in B. subtilis are partly controlled by peptides that accumulate in culture medium as cells grow to high density. We constructed two genes that encode mature forms of two different signaling molecules, the PhrA peptide that stimulates sporulation, and CSF, the competence- and sporulation-stimulating factor. Both pentapeptides are normally produced by secretion and processing of precursor molecules. The mature pentapeptides were functional when expressed inside the cell, indicating that they normally need to be imported to function. Furthermore, at physiological concentrations (10 nM), CSF was transported into the cell by the oligopeptide permease encoded by spo0K (opp). CSF was shown to have at least three different targets corresponding to its three activities: stimulating competence gene expression at low concentrations, and inhibiting competence gene expression and stimulating sporulation at high concentrations.en_US
dc.description.sponsorshipDamon Runyon Cancer Research Foundation (Fellowship DRG1384)en_US
dc.description.sponsorshipHoward Hughes Medical Institute (Predoctoral Fellowship)en_US
dc.description.sponsorshipLucille P. Markey Charitable Trusten_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Public Health Services Grant GM50895)en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/S0092-8674(00)80277-9en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceElsevier Open Archiveen_US
dc.titleAn Exported Peptide Functions Intracellularly to Contribute to Cell Density Signaling in B. subtilisen_US
dc.typeArticleen_US
dc.identifier.citationLazazzera, B. “An Exported Peptide Functions Intracellularly to Contribute to Cell Density Signaling in B. Subtilis.” Cell 89.6 (1997): 917–925. Copyright © 1997 Cell Pressen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorLazazzera, Beth A.en_US
dc.contributor.mitauthorSolomon, Jonathan M.en_US
dc.contributor.mitauthorGrossman, Alan D.en_US
dc.relation.journalCellen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLazazzera, Beth A.; Solomon, Jonathan M.; Grossman, Alan D.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-8235-7227
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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