dc.contributor.author | Lazazzera, Beth A. | |
dc.contributor.author | Solomon, Jonathan M. | |
dc.contributor.author | Grossman, Alan Davis | |
dc.date.accessioned | 2014-01-10T20:56:53Z | |
dc.date.available | 2014-01-10T20:56:53Z | |
dc.date.issued | 1997-06 | |
dc.date.submitted | 1997-05 | |
dc.identifier.issn | 00928674 | |
dc.identifier.issn | 1097-4172 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/83874 | |
dc.description.abstract | Competence development and sporulation in B. subtilis are partly controlled by peptides that accumulate in culture medium as cells grow to high density. We constructed two genes that encode mature forms of two different signaling molecules, the PhrA peptide that stimulates sporulation, and CSF, the competence- and sporulation-stimulating factor. Both pentapeptides are normally produced by secretion and processing of precursor molecules. The mature pentapeptides were functional when expressed inside the cell, indicating that they normally need to be imported to function. Furthermore, at physiological concentrations (10 nM), CSF was transported into the cell by the oligopeptide permease encoded by spo0K (opp). CSF was shown to have at least three different targets corresponding to its three activities: stimulating competence gene expression at low concentrations, and inhibiting competence gene expression and stimulating sporulation at high concentrations. | en_US |
dc.description.sponsorship | Damon Runyon Cancer Research Foundation (Fellowship DRG1384) | en_US |
dc.description.sponsorship | Howard Hughes Medical Institute (Predoctoral Fellowship) | en_US |
dc.description.sponsorship | Lucille P. Markey Charitable Trust | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Public Health Services Grant GM50895) | en_US |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1016/S0092-8674(00)80277-9 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | Elsevier Open Archive | en_US |
dc.title | An Exported Peptide Functions Intracellularly to Contribute to Cell Density Signaling in B. subtilis | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Lazazzera, B. “An Exported Peptide Functions Intracellularly to Contribute to Cell Density Signaling in B. Subtilis.” Cell 89.6 (1997): 917–925. Copyright © 1997 Cell Press | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.mitauthor | Lazazzera, Beth A. | en_US |
dc.contributor.mitauthor | Solomon, Jonathan M. | en_US |
dc.contributor.mitauthor | Grossman, Alan D. | en_US |
dc.relation.journal | Cell | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Lazazzera, Beth A.; Solomon, Jonathan M.; Grossman, Alan D. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-8235-7227 | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |