Show simple item record

dc.contributor.authorPulina, Maria V.
dc.contributor.authorHou, Shuan-Yu
dc.contributor.authorMittal, Ashok
dc.contributor.authorJulich, Dorthe
dc.contributor.authorWhittaker, Charlie A.
dc.contributor.authorHolley, Scott A.
dc.contributor.authorHynes, Richard O.
dc.contributor.authorAstrof, Sophie
dc.contributor.authorWhittaker, Charles A.
dc.date.accessioned2014-01-27T13:59:29Z
dc.date.available2014-01-27T13:59:29Z
dc.date.issued2011-04
dc.date.submitted2011-03
dc.identifier.issn00121606
dc.identifier.issn1095-564X
dc.identifier.urihttp://hdl.handle.net/1721.1/84535
dc.description.abstractStudies in Xenopus laevis suggested that cell-extracellular matrix (ECM) interactions regulate the development of the left–right axis of asymmetry; however, the identities of ECM components and their receptors important for this process have remained unknown. We discovered that FN is required for the establishment of the asymmetric gene expression pattern in early mouse embryos by regulating morphogenesis of the node, while cellular fates of the nodal cells, canonical Wnt and Shh signaling within the node were not perturbed by the absence of FN. FN is also required for the expression of Lefty 1/2 and activation of SMADs 2 and 3 at the floor plate, while cell fate specification of the notochord and the floor plate, as well as signaling within and between these two embryonic organizing centers remained intact in FN-null mutants. Furthermore, our experiments indicate that a major cell surface receptor for FN, integrin α5β1, is also required for the development of the left–right asymmetry, and that this requirement is evolutionarily conserved in fish and mice. Taken together, our studies demonstrate the requisite role for a structural ECM protein and its integrin receptor in the development of the left–right axis of asymmetry in vertebrates.en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.ydbio.2011.03.026en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceElsevier Open Archiveen_US
dc.titleEssential roles of fibronectin in the development of the left–right embryonic body planen_US
dc.typeArticleen_US
dc.identifier.citationPulina, Maria V., Shuan-Yu Hou, Ashok Mittal, Dorthe Julich, Charlie A. Whittaker, Scott A. Holley, Richard O. Hynes, and Sophie Astrof. “Essential roles of fibronectin in the development of the left–right embryonic body plan.” Developmental Biology 354, no. 2 (June 2011): 208-220. Copyright © 2011 Elsevier Inc.en_US
dc.contributor.departmentDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorWhittaker, Charles A.en_US
dc.contributor.mitauthorHynes, Richard O.en_US
dc.relation.journalDevelopmental Biologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsPulina, Maria V.; Hou, Shuan-Yu; Mittal, Ashok; Julich, Dorthe; Whittaker, Charlie A.; Holley, Scott A.; Hynes, Richard O.; Astrof, Sophieen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7603-8396
mit.licensePUBLISHER_POLICYen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record