dc.contributor.author | Nishino, Tatsuya | |
dc.contributor.author | Takeuchi, Kozo | |
dc.contributor.author | Gascoigne, Karen E. | |
dc.contributor.author | Suzuki, Aussie | |
dc.contributor.author | Hori, Tetsuya | |
dc.contributor.author | Oyama, Takuji | |
dc.contributor.author | Morikawa, Kosuke | |
dc.contributor.author | Fukagawa, Tatsuo | |
dc.contributor.author | Cheeseman, Iain M | |
dc.date.accessioned | 2014-01-27T17:22:03Z | |
dc.date.available | 2014-01-27T17:22:03Z | |
dc.date.issued | 2012-02 | |
dc.date.submitted | 2011-08 | |
dc.identifier.issn | 00928674 | |
dc.identifier.issn | 1097-4172 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/84581 | |
dc.description.abstract | The multiprotein kinetochore complex must assemble at a specific site on each chromosome to achieve accurate chromosome segregation. Defining the nature of the DNA-protein interactions that specify the position of the kinetochore and provide a scaffold for kinetochore formation remain key goals. Here, we demonstrate that the centromeric histone-fold-containing CENP-T-W and CENP-S-X complexes coassemble to form a stable CENP-T-W-S-X heterotetramer. High-resolution structural analysis of the individual complexes and the heterotetramer reveals similarity to other histone fold-containing complexes including canonical histones within a nucleosome. The CENP-T-W-S-X heterotetramer binds to and supercoils DNA. Mutants designed to compromise heterotetramerization or the DNA-protein contacts around the heterotetramer strongly reduce the DNA binding and supercoiling activities in vitro and compromise kinetochore assembly in vivo. These data suggest that the CENP-T-W-S-X complex forms a unique nucleosome-like structure to generate contacts with DNA, extending the “histone code” beyond canonical nucleosome proteins. | en_US |
dc.description.sponsorship | Kinship Foundation. Searle Scholars Program | en_US |
dc.description.sponsorship | National Institute of General Medical Sciences (U.S.) (Grant GM088313) | en_US |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1016/j.cell.2011.11.061 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | Elsevier Open Archive | en_US |
dc.title | CENP-T-W-S-X Forms a Unique Centromeric Chromatin Structure with a Histone-like Fold | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Nishino, Tatsuya, Kozo Takeuchi, Karen E. Gascoigne, Aussie Suzuki, Tetsuya Hori, Takuji Oyama, Kosuke Morikawa, Iain M. Cheeseman, and Tatsuo Fukagawa. “CENP-T-W-S-X Forms a Unique Centromeric Chromatin Structure with a Histone-like Fold.” Cell 148, no. 3 (February 2012): 487-501. Copyright © 2012 Elsevier Inc. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.mitauthor | Gascoigne, Karen E. | en_US |
dc.contributor.mitauthor | Cheeseman, Iain McPherson | en_US |
dc.relation.journal | Cell | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Nishino, Tatsuya; Takeuchi, Kozo; Gascoigne, Karen E.; Suzuki, Aussie; Hori, Tetsuya; Oyama, Takuji; Morikawa, Kosuke; Cheeseman, Iain M.; Fukagawa, Tatsuo | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-3829-5612 | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |