dc.contributor.author | Leung, Anthony | |
dc.contributor.author | Bhutkar, Arjun (AJ) | |
dc.contributor.author | Chang, Paul | |
dc.contributor.author | Vyas, Sejal Kamlesh | |
dc.contributor.author | Rood, Jennifer Evelyn | |
dc.contributor.author | Leung, Anthony | |
dc.contributor.author | Sharp, Phillip A. | |
dc.date.accessioned | 2014-01-27T18:31:08Z | |
dc.date.available | 2014-01-27T18:31:08Z | |
dc.date.issued | 2011-05 | |
dc.date.submitted | 2011-03 | |
dc.identifier.issn | 10972765 | |
dc.identifier.issn | 1097-4164 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/84591 | |
dc.description.abstract | Poly(ADP-ribose) is a major regulatory macromolecule in the nucleus, where it regulates transcription, chromosome structure, and DNA damage repair. Functions in the interphase cytoplasm are less understood. Here, we identify a requirement for poly(ADP-ribose) in the assembly of cytoplasmic stress granules, which accumulate RNA-binding proteins that regulate the translation and stability of mRNAs upon stress. We show that poly(ADP-ribose), six specific poly(ADP-ribose) polymerases, and two poly(ADP-ribose) glycohydrolase isoforms are stress granule components. A subset of stress granule proteins, including microRNA-binding Argonaute family members Ago1–4, are modified by poly(ADP-ribose), and such modification increases upon stress, a condition when both microRNA-mediated translational repression and microRNA-directed mRNA cleavage are relieved. Similar relief of repression is also observed upon overexpression of specific poly(ADP-ribose) polymerases or, conversely, upon knockdown of glycohydrolase. We conclude that poly(ADP-ribose) is a key regulator of posttranscriptional gene expression in the cytoplasm. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant RO1-CA133404) | en_US |
dc.description.sponsorship | National Cancer Institute (U.S.) (Grant PO1-CA42063) | en_US |
dc.description.sponsorship | National Cancer Institute (U.S.) (Cancer Center Support Core Grant P30-CA14051) | en_US |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1016/j.molcel.2011.04.015 | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | Elsevier Open Archive | en_US |
dc.title | Poly(ADP-Ribose) Regulates Stress Responses and MicroRNA Activity in the Cytoplasm | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Leung, Anthony K.L., Sejal Vyas, Jennifer E. Rood, Arjun Bhutkar, Phillip A. Sharp, and Paul Chang. “Poly(ADP-Ribose) Regulates Stress Responses and MicroRNA Activity in the Cytoplasm.” Molecular Cell 42, no. 4 (May 2011): 489-499. Copyright © 2011 Elsevier Inc. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | MIT Program in Writing & Humanistic Studies | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.contributor.mitauthor | Leung, Anthony K.L. | en_US |
dc.contributor.mitauthor | Vyas, Sejal Kamlesh | en_US |
dc.contributor.mitauthor | Rood, Jennifer Evelyn | en_US |
dc.contributor.mitauthor | Bhutkar, Arjun (AJ) | en_US |
dc.contributor.mitauthor | Sharp, Phillip A. | en_US |
dc.contributor.mitauthor | Chang, Paul | en_US |
dc.relation.journal | Molecular Cell | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Leung, Anthony K.L.; Vyas, Sejal; Rood, Jennifer E.; Bhutkar, Arjun; Sharp, Phillip A.; Chang, Paul | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-1465-1691 | |
dspace.mitauthor.error | true | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |