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dc.contributor.authorLeung, Anthony
dc.contributor.authorBhutkar, Arjun (AJ)
dc.contributor.authorChang, Paul
dc.contributor.authorVyas, Sejal Kamlesh
dc.contributor.authorRood, Jennifer Evelyn
dc.contributor.authorLeung, Anthony
dc.contributor.authorSharp, Phillip A.
dc.date.accessioned2014-01-27T18:31:08Z
dc.date.available2014-01-27T18:31:08Z
dc.date.issued2011-05
dc.date.submitted2011-03
dc.identifier.issn10972765
dc.identifier.issn1097-4164
dc.identifier.urihttp://hdl.handle.net/1721.1/84591
dc.description.abstractPoly(ADP-ribose) is a major regulatory macromolecule in the nucleus, where it regulates transcription, chromosome structure, and DNA damage repair. Functions in the interphase cytoplasm are less understood. Here, we identify a requirement for poly(ADP-ribose) in the assembly of cytoplasmic stress granules, which accumulate RNA-binding proteins that regulate the translation and stability of mRNAs upon stress. We show that poly(ADP-ribose), six specific poly(ADP-ribose) polymerases, and two poly(ADP-ribose) glycohydrolase isoforms are stress granule components. A subset of stress granule proteins, including microRNA-binding Argonaute family members Ago1–4, are modified by poly(ADP-ribose), and such modification increases upon stress, a condition when both microRNA-mediated translational repression and microRNA-directed mRNA cleavage are relieved. Similar relief of repression is also observed upon overexpression of specific poly(ADP-ribose) polymerases or, conversely, upon knockdown of glycohydrolase. We conclude that poly(ADP-ribose) is a key regulator of posttranscriptional gene expression in the cytoplasm.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant RO1-CA133404)en_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (Grant PO1-CA42063)en_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (Cancer Center Support Core Grant P30-CA14051)en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.molcel.2011.04.015en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceElsevier Open Archiveen_US
dc.titlePoly(ADP-Ribose) Regulates Stress Responses and MicroRNA Activity in the Cytoplasmen_US
dc.typeArticleen_US
dc.identifier.citationLeung, Anthony K.L., Sejal Vyas, Jennifer E. Rood, Arjun Bhutkar, Phillip A. Sharp, and Paul Chang. “Poly(ADP-Ribose) Regulates Stress Responses and MicroRNA Activity in the Cytoplasm.” Molecular Cell 42, no. 4 (May 2011): 489-499. Copyright © 2011 Elsevier Inc.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentMIT Program in Writing & Humanistic Studiesen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorLeung, Anthony K.L.en_US
dc.contributor.mitauthorVyas, Sejal Kamleshen_US
dc.contributor.mitauthorRood, Jennifer Evelynen_US
dc.contributor.mitauthorBhutkar, Arjun (AJ)en_US
dc.contributor.mitauthorSharp, Phillip A.en_US
dc.contributor.mitauthorChang, Paulen_US
dc.relation.journalMolecular Cellen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLeung, Anthony K.L.; Vyas, Sejal; Rood, Jennifer E.; Bhutkar, Arjun; Sharp, Phillip A.; Chang, Paulen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-1465-1691
dspace.mitauthor.errortrue
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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