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dc.contributor.authorLorestani, Alexander
dc.contributor.authorIvey, F. Douglas
dc.contributor.authorThirugnanam, Sivasakthivel
dc.contributor.authorBusby, Michele A.
dc.contributor.authorMarth, Gabor T.
dc.contributor.authorGubbels, Marc-Jan
dc.contributor.authorCheeseman, Iain M
dc.date.accessioned2014-01-27T18:49:14Z
dc.date.available2014-01-27T18:49:14Z
dc.date.issued2012-10
dc.date.submitted2012-09
dc.identifier.issn19493584
dc.identifier.issn1949-3592
dc.identifier.urihttp://hdl.handle.net/1721.1/84593
dc.description.abstractThe basal complex in Toxoplasma functions as the contractile ring in the cell division process. Basal complex contraction tapers the daughter cytoskeleton toward the basal end and is required for daughter segregation. We have previously shown that the protein MORN1 is essential for basal complex assembly and likely acts as a scaffolding protein. To further our understanding of the basal complex, we combined subcellular fractionation with an affinity purification of the MORN1 complex and identified its protein composition. We identified two new components of the basal complex, one of which uniquely associated with the basal complex in mature parasites, the first of its kind. In addition, we identified several other novel cytoskeleton proteins with different spatiotemporal dynamics throughout cell division. Since many of these proteins are unique to Apicomplexa this study significantly contributes to the annotation of their unique cytoskeleton. Furthermore, we show that G-actin binding protein TgCAP is localized at the apical cap region in intracellular parasites, but quickly redistributes to a cytoplasmic localization pattern upon egress.en_US
dc.description.sponsorshipNew England Regional Center of Excellence for Biodefense and Emerging Infectious Diseases (National Institutes of Health (U.S.) Grant R01AI081924)en_US
dc.description.sponsorshipNew England Regional Center of Excellence for Biodefense and Emerging Infectious Diseases (National Institutes of Health (U.S.) Grant U54AI057159)en_US
dc.language.isoen_US
dc.publisherWiley Blackwellen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/cm.21077en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourcePMCen_US
dc.titleTargeted proteomic dissection of Toxoplasma cytoskeleton sub-compartments using MORN1en_US
dc.typeArticleen_US
dc.identifier.citationLorestani, Alexander, F. Douglas Ivey, Sivasakthivel Thirugnanam, Michele A. Busby, Gabor T. Marth, Iain M. Cheeseman, and Marc-Jan Gubbels. “Targeted proteomic dissection of Toxoplasma cytoskeleton sub-compartments using MORN1.” Cytoskeleton 69, no. 12 (December 11, 2012): 1069-1085.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorCheeseman, Iain McPhersonen_US
dc.relation.journalCytoskeletonen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLorestani, Alexander; Ivey, F. Douglas; Thirugnanam, Sivasakthivel; Busby, Michele A.; Marth, Gabor T.; Cheeseman, Iain M.; Gubbels, Marc-Janen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-3829-5612
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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