Show simple item record

dc.contributor.authorDuPage, Michel J.
dc.contributor.authorBrauneis, Alison L.
dc.contributor.authorJacks, Tyler E.
dc.date.accessioned2014-01-27T19:35:16Z
dc.date.available2014-01-27T19:35:16Z
dc.date.issued2009-06
dc.identifier.issn1754-2189
dc.identifier.issn1750-2799
dc.identifier.urihttp://hdl.handle.net/1721.1/84599
dc.description.abstractThe development of animal models of lung cancer is critical to our understanding and treatment of the human disease. Conditional mouse models provide new opportunities for testing novel chemopreventatives, therapeutics and screening methods that are not possible with cultured cell lines or xenograft models. This protocol describes how to initiate tumors in two conditional genetic models of human non-small cell lung cancer (NSCLC) using the activation of oncogenic K-ras alone or in combination with the loss of function of p53. We discuss methods for sporadic expression of Cre in the lungs through engineered adenovirus or lentivirus, and provide a detailed protocol for the administration of the virus by intranasal inhalation or intratracheal intubation. The protocol requires 1–5 min per mouse with an additional 30–45 min to set-up and allow for the recovery of mice from anesthesia. Mice may be analyzed for tumor formation and progression starting 2–3 weeks after infection.en_US
dc.description.sponsorshipHoward Hughes Medical Instituteen_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (Cancer Center Support Grant)en_US
dc.description.sponsorshipMassachusetts Institute of Technology. Ludwig Center for Molecular Oncologyen_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nprot.2009.95en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourcePMCen_US
dc.titleConditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinaseen_US
dc.typeArticleen_US
dc.identifier.citationDuPage, Michel, Alison L Dooley, and Tyler Jacks. “Conditional mouse lung cancer models using adenoviral or lentiviral delivery of Cre recombinase.” Nature Protocols 4, no. 8 (June 25, 2009): 1064-1072.en_US
dc.contributor.departmentmove to dc.description.sponsorshipen_US
dc.contributor.departmentDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorDuPage, Michel J.en_US
dc.contributor.mitauthorBrauneis, Alison L.en_US
dc.contributor.mitauthorJacks, Tyler E.en_US
dc.relation.journalNature Protocolsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsDuPage, Michel; Dooley, Alison L; Jacks, Tyleren_US
dc.identifier.orcidhttps://orcid.org/0000-0001-5785-8911
dc.identifier.orcidhttps://orcid.org/0000-0002-1846-604X
dspace.mitauthor.errortrue
mit.licenseOPEN_ACCESS_POLICYen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record