dc.contributor.author | Halfmann, Randal Arthur | |
dc.contributor.author | Wright, Jessica R. | |
dc.contributor.author | Alberti, Simon | |
dc.contributor.author | Lindquist, Susan | |
dc.contributor.author | Rexach, Michael | |
dc.date.accessioned | 2014-02-14T18:18:58Z | |
dc.date.available | 2014-02-14T18:18:58Z | |
dc.date.issued | 2012-09 | |
dc.date.submitted | 2012-03 | |
dc.identifier.issn | 1933-6896 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/84962 | |
dc.description.abstract | The self-assembly of proteins into higher order structures is both central to normal biology and a dominant force in disease. Certain glutamine/asparagine (Q/N)-rich proteins in the budding yeast Saccharomyces cerevisiae assemble into self-replicating amyloid-like protein polymers, or prions, that act as genetic elements in an entirely protein-based system of inheritance. The nuclear pore complex (NPC) contains multiple Q/N-rich proteins whose self-assembly has also been proposed to underlie structural and functional properties of the NPC. Here we show that an essential sequence feature of these proteins—repeating GLFG motifs—strongly promotes their self-assembly into amyloids with characteristics of prions. Furthermore, we demonstrate that Nup100 can form bona fide prions, thus establishing a previously undiscovered ability of yeast GLFG nucleoporins to adopt this conformational state in vivo. | en_US |
dc.description.sponsorship | G. Harold and Leila Y. Mathers Foundation | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (grant GM061900) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (grant GM007520) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (grant GM25874) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Director’s Early Independence Award, DP5-OD009152-01) | en_US |
dc.description.sponsorship | Howard Hughes Medical Institute (Investigator) | en_US |
dc.description.sponsorship | University of Texas Southwestern Medical Center at Dallas (Frank and Sara McKnight Fellow) | en_US |
dc.language.iso | en_US | |
dc.publisher | Landes Bioscience | en_US |
dc.relation.isversionof | http://dx.doi.org/10.4161/pri.20199 | en_US |
dc.rights | Creative Commons Attribution | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/ | en_US |
dc.source | Prion | en_US |
dc.title | Prion formation by a yeast GLFG nucleoporin | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Halfmann, Randal, Jessica R. Wright, Simon Alberti, Susan Lindquist, and Michael Rexach. “Prion formation by a yeast GLFG nucleoporin.” Prion 6, no. 4 (September 1, 2012): 391-399. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Whitehead Institute for Biomedical Research | en_US |
dc.contributor.mitauthor | Halfmann, Randal Arthur | en_US |
dc.contributor.mitauthor | Alberti, Simon | en_US |
dc.contributor.mitauthor | Lindquist, Susan | en_US |
dc.relation.journal | Prion | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Halfmann, Randal; Wright, Jessica R.; Alberti, Simon; Lindquist, Susan; Rexach, Michael | en_US |
dc.identifier.orcid | https://orcid.org/0000-0003-1307-882X | |
dspace.mitauthor.error | true | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |