The Xist lncRNA Exploits Three-Dimensional Genome Architecture to Spread Across the X Chromosome
Author(s)Engreitz, Jesse Michael; Lander, Eric S.; Pandya-Jones, Amy; McDonel, Patrick; Shishkin, Alexander; Sirokman, Klara; Surka, Christine; Kadri, Sabah; Xing, Jeffrey; Goren, Alon; Plath, Kathrin; Guttman, Mitchell; ... Show more Show less
MetadataShow full item record
Many large noncoding RNAs (lncRNAs) regulate chromatin, but the mechanisms by which they localize to genomic targets remain unexplored. We investigated the localization mechanisms of the Xist lncRNA during X-chromosome inactivation (XCI), a paradigm of lncRNA-mediated chromatin regulation. During the maintenance of XCI, Xist binds broadly across the X chromosome. During initiation of XCI, Xist initially transfers to distal regions across the X chromosome that are not defined by specific sequences. Instead, Xist identifies these regions by exploiting the three-dimensional conformation of the X chromosome. Xist requires its silencing domain to spread across actively transcribed regions and thereby access the entire chromosome. These findings suggest a model in which Xist coats the X chromosome by searching in three dimensions, modifying chromosome structure, and spreading to newly accessible locations.
DepartmentWhitaker College of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Biology
American Association for the Advancement of Science (AAAS)
Engreitz, J. M., A. Pandya-Jones, P. McDonel, A. Shishkin, K. Sirokman, C. Surka, S. Kadri, et al. “The Xist lncRNA Exploits Three-Dimensional Genome Architecture to Spread Across the X Chromosome.” Science 341, no. 6147 (August 16, 2013): 1237973–1237973.
Author's final manuscript