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dc.contributor.authorGuan, Zhuo
dc.contributor.authorBuhl, Lauren Kaye
dc.contributor.authorQuinn, William G.
dc.contributor.authorLittleton, J. Troy
dc.date.accessioned2014-03-14T14:36:01Z
dc.date.available2014-03-14T14:36:01Z
dc.date.issued2011-03
dc.identifier.issn1072-0502
dc.identifier.issn1549-5485
dc.identifier.urihttp://hdl.handle.net/1721.1/85615
dc.description.abstractGenetic studies in Drosophila have revealed two separable long-term memory pathways defined as anesthesia-resistant memory (ARM) and long-lasting long-term memory (LLTM). ARM is disrupted in radish (rsh) mutants, whereas LLTM requires CREB-dependent protein synthesis. Although the downstream effectors of ARM and LLTM are distinct, pathways leading to these forms of memory may share the cAMP cascade critical for associative learning. Dunce, which encodes a cAMP-specific phosphodiesterase, and rutabaga, which encodes an adenylyl cyclase, both disrupt short-term memory. Amnesiac encodes a pituitary adenylyl cyclase-activating peptide homolog and is required for middle-term memory. Here, we demonstrate that the Radish protein localizes to the cytoplasm and nucleus and is a PKA phosphorylation target in vitro. To characterize how these plasticity pathways may manifest at the synaptic level, we assayed synaptic connectivity and performed an expression analysis to detect altered transcriptional networks in rutabaga, dunce, amnesiac, and radish mutants. All four mutants disrupt specific aspects of synaptic connectivity at larval neuromuscular junctions (NMJs). Genome-wide DNA microarray analysis revealed ∼375 transcripts that are altered in these mutants, suggesting defects in multiple neuronal signaling pathways. In particular, the transcriptional target Lapsyn, which encodes a leucine-rich repeat cell adhesion protein, localizes to synapses and regulates synaptic growth. This analysis provides insights into the Radish-dependent ARM pathway and novel transcriptional targets that may contribute to memory processing in Drosophila.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant no. NS43244)en_US
dc.language.isoen_US
dc.publisherCold Spring Harbor Laboratory Pressen_US
dc.relation.isversionofhttp://dx.doi.org/10.1101/lm.2027111en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourceLittletonen_US
dc.titleAltered gene regulation and synaptic morphology in Drosophila learning and memory mutantsen_US
dc.typeArticleen_US
dc.identifier.citationGuan, Z., L. K. Buhl, W. G. Quinn, and J. T. Littleton. “Altered Gene Regulation and Synaptic Morphology in Drosophila Learning and Memory Mutants.” Learning & Memory 18, no. 4 (March 21, 2011): 191–206.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentPicower Institute for Learning and Memoryen_US
dc.contributor.approverLittleton, J. Troyen_US
dc.contributor.mitauthorGuan, Zhuoen_US
dc.contributor.mitauthorBuhl, Lauren Kayeen_US
dc.contributor.mitauthorQuinn, William G.en_US
dc.contributor.mitauthorLittleton, J. Troyen_US
dc.relation.journalLearning & Memoryen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsGuan, Z.; Buhl, L. K.; Quinn, W. G.; Littleton, J. T.en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-5576-2887
dc.identifier.orcidhttps://orcid.org/0000-0002-4314-0519
dspace.mitauthor.errortrue
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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