Next-generation transgenic mice for optogenetic analysis of neural circuits

Author(s)
Asrican, Brent; Augustine, George J.; Berglund, Ken; Chen, Susu; Chow, Nick; ... Show more
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Abstract
Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.
Date issued
2013-11
URI
http://hdl.handle.net/1721.1/85681
Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; McGovern Institute for Brain Research at MIT
Journal
Frontiers in Neural Circuits
Publisher
Frontiers Research Foundation
Citation
Asrican, Brent, George J. Augustine, Ken Berglund, Susu Chen, Nick Chow, Karl Deisseroth, Guoping Feng, et al. “Next-Generation Transgenic Mice for Optogenetic Analysis of Neural Circuits.” Front. Neural Circuits 7 (2013).
Version: Final published version
ISSN
1662-5110
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