Next-generation transgenic mice for optogenetic analysis of neural circuits
Author(s)Asrican, Brent; Augustine, George J.; Berglund, Ken; Chen, Susu; Chow, Nick; Deisseroth, Karl; Feng, Guoping; Gloss, Bernd; Hira, Riichiro; Hoffmann, Carolin; Kasai, Haruo; Katarya, Malvika; Kim, Jinsook; Kudolo, John; Lee, Li Ming; Lo, Shun Qiang; Mancuso, James; Matsuzaki, Masanori; Nakajima, Ryuichi; Qiu, Li; Tan, Gregory; Tang, Yanxia; Tsuda, Sachiko; Wen, Lei; Zhang, Xuying; Zhao, Shengli; Ting, Jonathan Thomas; ... Show more Show less
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Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.
DepartmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciences; McGovern Institute for Brain Research at MIT
Frontiers in Neural Circuits
Frontiers Research Foundation
Asrican, Brent, George J. Augustine, Ken Berglund, Susu Chen, Nick Chow, Karl Deisseroth, Guoping Feng, et al. “Next-Generation Transgenic Mice for Optogenetic Analysis of Neural Circuits.” Front. Neural Circuits 7 (2013).
Final published version