MIT Libraries logoDSpace@MIT

MIT
View Item 
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons

Author(s)
Logan, David J.; Saulnier, Jessica; Lam, Daniel; Johnson, Caroline; Root, David E.; Carpenter, Anne E.; Sabatini, Bernardo L.; Nieland, Thomas J; ... Show more Show less
Thumbnail
DownloadNieland-2014-High Content Image A.pdf (3.250Mb)
PUBLISHER_CC

Publisher with Creative Commons License

Creative Commons Attribution

Terms of use
Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/
Metadata
Show full item record
Abstract
The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neuropsychiatric disorders, such as Alzheimer's disease, intellectual disability, and autism spectrum disorders. Many genes controlling synaptogenesis have been identified, but lack of facile experimental systems has made systematic discovery of regulators of synaptogenesis challenging. Thus, we created a high-throughput platform to study excitatory and inhibitory synapse development in primary neuronal cultures and used a lentiviral RNA interference library to identify novel regulators of synapse formation. This methodology is broadly applicable for high-throughput screening of genes and drugs that may rescue or improve synaptic dysfunction associated with cognitive function and neurological disorders.
Date issued
2014-03
URI
http://hdl.handle.net/1721.1/86091
Department
Massachusetts Institute of Technology. Department of Biology
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Nieland, Thomas J. F., David J. Logan, Jessica Saulnier, Daniel Lam, Caroline Johnson, David E. Root, Anne E. Carpenter, and Bernardo L. Sabatini. “High Content Image Analysis Identifies Novel Regulators of Synaptogenesis in a High-Throughput RNAi Screen of Primary Neurons.” Edited by Lin Mei. PLoS ONE 9, no. 3 (March 14, 2014): e91744.
Version: Final published version
ISSN
1932-6203

Collections
  • MIT Open Access Articles

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

Login

Statistics

OA StatisticsStatistics by CountryStatistics by Department
MIT Libraries
PrivacyPermissionsAccessibilityContact us
MIT
Content created by the MIT Libraries, CC BY-NC unless otherwise noted. Notify us about copyright concerns.