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dc.contributor.authorShan, Jing
dc.contributor.authorRoss, Nathan T.
dc.contributor.authorLogan, David J.
dc.contributor.authorThomas, David
dc.contributor.authorDuncan, Stephen A.
dc.contributor.authorNorth, Trista E.
dc.contributor.authorGoessling, Wolfram
dc.contributor.authorCarpenter, Anne E.
dc.contributor.authorSchwartz, Robert E.
dc.contributor.authorBhatia, Sangeeta N
dc.date.accessioned2014-04-11T17:04:24Z
dc.date.available2014-04-11T17:04:24Z
dc.date.issued2013-06
dc.date.submitted2012-12
dc.identifier.issn1552-4450
dc.identifier.issn1552-4469
dc.identifier.urihttp://hdl.handle.net/1721.1/86114
dc.description.abstractCell-based therapies hold the potential to alleviate the growing burden of liver diseases. Such therapies require human hepatocytes, which, within the stromal context of the liver, are capable of many rounds of replication. However, this ability is lost ex vivo, and human hepatocyte sourcing has limited many fields of research for decades. Here we developed a high-throughput screening platform for primary human hepatocytes to identify small molecules in two different classes that can be used to generate renewable sources of functional human hepatocytes. The first class induced functional proliferation of primary human hepatocytes in vitro. The second class enhanced hepatocyte functions and promoted the differentiation of induced pluripotent stem cell–derived hepatocytes toward a more mature phenotype than what was previously obtainable. The identification of these small molecules can help address a major challenge affecting many facets of liver research and may lead to the development of new therapeutics for liver diseases.en_US
dc.description.sponsorshipBroad Institute of MIT and Harvard (Scientific Planning and Allocation of Resources Committee Grant)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant NIH R01-DK065152)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant NIH R01-DK56966)en_US
dc.language.isoen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nchembio.1270en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleIdentification of small molecules for human hepatocyte expansion and iPS differentiationen_US
dc.typeArticleen_US
dc.identifier.citationShan, Jing, Robert E Schwartz, Nathan T Ross, David J Logan, David Thomas, Stephen A Duncan, Trista E North, Wolfram Goessling, Anne E Carpenter, and Sangeeta N Bhatia. “Identification of Small Molecules for Human Hepatocyte Expansion and iPS Differentiation.” Nat Chem Biol 9, no. 8 (June 2, 2013): 514–520.en_US
dc.contributor.departmentWhitaker College of Health Sciences and Technologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering & Scienceen_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorShan, Jingen_US
dc.contributor.mitauthorSchwartz, Roberten_US
dc.contributor.mitauthorBhatia, Sangeeta N.en_US
dc.relation.journalNature Chemical Biologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsShan, Jing; Schwartz, Robert E; Ross, Nathan T; Logan, David J; Thomas, David; Duncan, Stephen A; North, Trista E; Goessling, Wolfram; Carpenter, Anne E; Bhatia, Sangeeta Nen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-0590-9937
dc.identifier.orcidhttps://orcid.org/0000-0002-1293-2097
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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