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dc.contributor.authorHuang, Sha
dc.contributor.authorUndisz, Andreas
dc.contributor.authorDiez Silva, Monica
dc.contributor.authorBow, Hansen
dc.contributor.authorDao, Ming
dc.contributor.authorHan, Jongyoon
dc.date.accessioned2014-05-02T17:09:00Z
dc.date.available2014-05-02T17:09:00Z
dc.date.issued2012-11
dc.date.submitted2012-06
dc.identifier.issn1757-9694
dc.identifier.issn1757-9708
dc.identifier.urihttp://hdl.handle.net/1721.1/86377
dc.description.abstractArtesunate (ART) is widely used for the treatment of malaria, but the mechanisms of its effects on parasitized red blood cells (RBCs) are not fully understood. We investigated ART's influence on the dynamic deformability of ring-stage Plasmodium falciparum infected red blood cells (iRBCs) in order to elucidate its role in cellular mechanobiology. The dynamic deformability of RBCs was measured by passing them through a microfluidic device with repeated bottleneck structures. The quasi-static deformability measurement was performed using micropipette aspiration. After ART treatment, microfluidic experiments showed 50% decrease in iRBC transit velocity whereas only small (~10%) velocity reduction was observed among uninfected RBCs (uRBCs). Micropipette aspiration also revealed ART-induced stiffening in RBC membranes. These results demonstrate, for the first time, that ART reduces the dynamic and quasi-static RBC deformability, which may subsequently influence blood circulation through the microvasculature and spleen cordal meshwork, thus adding a new aspect to artesunate's mechanism of action.en_US
dc.description.sponsorshipSingapore-MIT Alliance for Research and Technology Centeren_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R01 HL094270-01A1)en_US
dc.language.isoen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.isversionofhttp://dx.doi.org/10.1039/c2ib20161een_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleDynamic deformability of Plasmodium falciparum-infected erythrocytes exposed to artesunate in vitroen_US
dc.typeArticleen_US
dc.identifier.citationHuang, Sha, Andreas Undisz, Monica Diez-Silva, Hansen Bow, Ming Dao, and Jongyoon Han. “Dynamic Deformability of Plasmodium Falciparum-Infected Erythrocytes Exposed to Artesunate in Vitro.” Integr. Biol. 5, no. 2 (2013): 414.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Materials Science and Engineeringen_US
dc.contributor.mitauthorHuang, Shaen_US
dc.contributor.mitauthorUndisz, Andreasen_US
dc.contributor.mitauthorDiez Silva, Monicaen_US
dc.contributor.mitauthorBow, Hansenen_US
dc.contributor.mitauthorDao, Mingen_US
dc.contributor.mitauthorHan, Jongyoonen_US
dc.relation.journalIntegrative Biologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsHuang, Sha; Undisz, Andreas; Diez-Silva, Monica; Bow, Hansen; Dao, Ming; Han, Jongyoonen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7215-1439
dspace.mitauthor.errortrue
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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