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dc.contributor.authorShafiee, Hadi
dc.contributor.authorLidstone, Erich A.
dc.contributor.authorJahangir, Muntasir
dc.contributor.authorInci, Fatih
dc.contributor.authorHanhauser, Emily
dc.contributor.authorHenrich, Timothy J.
dc.contributor.authorKuritzkes, Daniel R.
dc.contributor.authorCunningham, Brian T.
dc.contributor.authorDemirci, Utkan
dc.date.accessioned2014-05-30T17:40:53Z
dc.date.available2014-05-30T17:40:53Z
dc.date.issued2014-02
dc.date.submitted2013-09
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/87591
dc.description.abstractDetecting and quantifying biomarkers and viruses in biological samples have broad applications in early disease diagnosis and treatment monitoring. We have demonstrated a label-free optical sensing mechanism using nanostructured photonic crystals (PC) to capture and quantify intact viruses (HIV-1) from biologically relevant samples. The nanostructured surface of the PC biosensor resonantly reflects a narrow wavelength band during illumination with a broadband light source. Surface-adsorbed biotarget induces a shift in the resonant Peak Wavelength Value (PWV) that is detectable with <10 pm wavelength resolution, enabling detection of both biomolecular layers and small number of viruses that sparsely populate the transducer surface. We have successfully captured and detected HIV-1 in serum and phosphate buffered saline (PBS) samples with viral loads ranging from 10[superscript 4] to 10[superscript 8] copies/mL. The surface density of immobilized biomolecular layers used in the sensor functionalization process, including 3-mercaptopropyltrimethoxysilane (3-MPS), N-gamma-Maleimidobutyryl-oxysuccinimide ester (GMBS), NeutrAvidin, anti-gp120, and bovine serum albumin (BSA) were also quantified by the PC biosensor.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Award R01AI093282)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Award R01AI081534)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Award R21AI087107)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Award F32AI102590)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep04116en_US
dc.rightsCreative Commons Attribution-Noncommercialen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0en_US
dc.sourceNature Publishing Groupen_US
dc.titleNanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurementen_US
dc.typeArticleen_US
dc.identifier.citationShafiee, Hadi, Erich A. Lidstone, Muntasir Jahangir, Fatih Inci, Emily Hanhauser, Timothy J. Henrich, Daniel R. Kuritzkes, Brian T. Cunningham, and Utkan Demirci. “Nanostructured Optical Photonic Crystal Biosensor for HIV Viral Load Measurement.” Sci. Rep. 4 (February 28, 2014).en_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.mitauthorDemirci, Utkanen_US
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsShafiee, Hadi; Lidstone, Erich A.; Jahangir, Muntasir; Inci, Fatih; Hanhauser, Emily; Henrich, Timothy J.; Kuritzkes, Daniel R.; Cunningham, Brian T.; Demirci, Utkanen_US
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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