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dc.contributor.authorLaiho, Lily H.
dc.contributor.authorPelet, Serge
dc.contributor.authorHancewicz, Thomas M.
dc.contributor.authorKaplan, Peter D.
dc.contributor.authorSo, Peter T. C.
dc.date.accessioned2014-06-05T13:54:59Z
dc.date.available2014-06-05T13:54:59Z
dc.date.issued2005-03
dc.date.submitted2004-10
dc.identifier.issn10833668
dc.identifier.issn1560-2281
dc.identifier.urihttp://hdl.handle.net/1721.1/87642
dc.description.abstractSpectral resolved tissue imaging has a broad range of biomedical applications such as the minimally invasive diagnosis of diseases and the study of wound healing and tissue engineering processes. Two-photon microscopy imaging of endogenous fluorescence has been shown to be a powerful method for the quantification of tissue structure and biochemistry. While two-photon excited autofluorescence is observed ubiquitously, the identities and distributions of endogenous fluorophores have not been completely characterized in most tissues. We develop an image-guided spectral analysis method to analyze the distribution of fluorophores in human skin from 3-D resolved two-photon images. We identify five factors that contribute to most of the luminescence signals from human skin. Luminescence species identified include tryptophan, NAD(P)H, melanin, and elastin, which are autofluorescent, and collagen that contributes to a second harmonic signal.en_US
dc.description.sponsorshipUnilever (Firm)en_US
dc.description.sponsorshipAmerican Cancer Society (RPG-98-058-01-CCE)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (R33 CA091354-01A1)en_US
dc.language.isoen_US
dc.publisherSPIEen_US
dc.relation.isversionofhttp://dx.doi.org/10.1117/1.1891370en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceSPIEen_US
dc.titleTwo-photon 3-D mapping of ex vivo human skin endogenous fluorescence species based on fluorescence emission spectraen_US
dc.typeArticleen_US
dc.identifier.citationLaiho, Lily H., Serge Pelet, Thomas M. Hancewicz, Peter D. Kaplan, and Peter T. C. So. “Two-Photon 3-D Mapping of Ex Vivo Human Skin Endogenous Fluorescence Species Based on Fluorescence Emission Spectra.” Journal of Biomedical Optics 10, no. 2 (2005): 024016. © 2005 Society of Photo-Optical Instrumentation Engineersen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.mitauthorLaiho, Lily H.en_US
dc.contributor.mitauthorPelet, Sergeen_US
dc.contributor.mitauthorSo, Peter T. C.en_US
dc.relation.journalJournal of Biomedical Opticsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLaiho, Lily H.; Pelet, Serge; Hancewicz, Thomas M.; Kaplan, Peter D.; So, Peter T. C.en_US
dc.identifier.orcidhttps://orcid.org/0000-0003-4698-6488
mit.licensePUBLISHER_POLICYen_US


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