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dc.contributor.authorTawakol, Ahmed
dc.contributor.authorCastano, Ana P.
dc.contributor.authorAnatelli, Florencia
dc.contributor.authorBashian, Gregory
dc.contributor.authorStern, Jeremy
dc.contributor.authorZahra, Touqir
dc.contributor.authorGad, Faten
dc.contributor.authorChirico, Stephanie
dc.contributor.authorAhmadi, Atosa
dc.contributor.authorFischman, Alan J.
dc.contributor.authorMuller, James E.
dc.contributor.authorHamblin, Michael R.
dc.date.accessioned2014-06-05T17:29:03Z
dc.date.available2014-06-05T17:29:03Z
dc.date.issued2006-04
dc.date.submitted2005-10
dc.identifier.issn10833668
dc.identifier.issn1560-2281
dc.identifier.urihttp://hdl.handle.net/1721.1/87656
dc.description.abstractWe have previously shown that a conjugate (MA-ce6) between maleylated serum albumin and the photosensitizer chlorin(e6) (ce6) is targeted in vitro to macrophages via class A scavenger receptors. We now report on the ability of this conjugate to localize in macrophage-rich atherosclerotic plaques in vivo. Both the conjugate and the free photosensitizer ce6 are studied after injection into New Zealand White rabbits that are rendered atherosclerotic by a combination of aortic endothelial injury and cholesterol feeding into normal rabbits. Rabbits are sacrificed at 6 and 24 h after injection and intravascular fluorescence spectroscopy is carried out by fiber-based fluorimetry in intact blood-filled arteries. Surface spectrofluorimetry of numbered excised aortic segments together with injured and normal iliac arteries is carried out, and quantified ce6 content by subsequent extraction and quantitative fluorescence determination of the arterial segments and also of nontarget organs. There is good agreement between the various techniques for quantifying ce6 localization, and high contrast between arteries from atherosclerotic and normal rabbits is obtained. Fluorescence correlates with the highest burden of plaque in the aorta and the injured iliac artery. The highest accumulation in plaques is obtained using MA-ce6 at 24 h. Free ce6 gives better accumulation at 6 h compared to 24 h. The liver, spleen, lung, and gall bladder have the highest uptake in nontarget organs. Macrophage-targeted photosensitizer conjugates may have applications in both detecting and treating inflamed vulnerable plaque.en_US
dc.description.sponsorshipCenter for Integration of Medicine and Innovative Technology (Grant DAMD 17-02-2-0006)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant CA/AI838801)en_US
dc.language.isoen_US
dc.publisherSPIEen_US
dc.relation.isversionofhttp://dx.doi.org/10.1117/1.2186039en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceSPIEen_US
dc.titlePhotosensitizer delivery to vulnerable atherosclerotic plaque: comparison of macrophage-targeted conjugate versus free chlorine(e6)en_US
dc.typeArticleen_US
dc.identifier.citationTawakol, Ahmed, Ana P. Castano, Florencia Anatelli, Gregory Bashian, Jeremy Stern, Touqir Zahra, Faten Gad, et al. “Photosensitizer Delivery to Vulnerable Atherosclerotic Plaque: Comparison of Macrophage-Targeted Conjugate Versus Free Chlorine(e6).” Journal of Biomedical Optics 11, no. 2 (2006): 021008. © 2006 Society of Photo-Optical Instrumentation Engineersen_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.mitauthorCastano, Ana P.en_US
dc.contributor.mitauthorHamblin, Michael R.en_US
dc.relation.journalJournal of Biomedical Opticsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsTawakol, Ahmed; Castano, Ana P.; Anatelli, Florencia; Bashian, Gregory; Stern, Jeremy; Zahra, Touqir; Gad, Faten; Chirico, Stephanie; Ahmadi, Atosa; Fischman, Alan J.; Muller, James E.; Hamblin, Michael R.en_US
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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