Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function
Author(s)Zhao, Shengli; Ting, Jonathan Thomas; Atallah, Hicham; Qiu, Li; Tan, Jie; Gloss, Bernd; Augustine, George J.; Luo, Minmin; Deisseroth, Karl; Graybiel, Ann M.; Feng, Guoping; ... Show more Show less
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Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function and dysfunction. We used a bacterial artificial chromosome (BAC) transgenic strategy to express the H134R variant of channelrhodopsin-2, ChR2(H134R), under the control of cell type–specific promoter elements. We performed an extensive functional characterization of the newly established VGAT-ChR2(H134R)-EYFP, ChAT-ChR2(H134R)-EYFP, Tph2-ChR2(H134R)-EYFP and Pvalb(H134R)-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action-potential firing of GABAergic, cholinergic, serotonergic and parvalbumin-expressing neuron subsets using blue light. This resource of cell type–specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior.
Available in PMC 2011 October 12.
DepartmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciences; McGovern Institute for Brain Research at MIT
Nature Publishing Group
Zhao, Shengli, Jonathan T Ting, Hisham E Atallah, Li Qiu, Jie Tan, Bernd Gloss, George J Augustine, et al. “Cell Type–specific Channelrhodopsin-2 Transgenic Mice for Optogenetic Dissection of Neural Circuitry Function.” Nature Methods 8, no. 9 (August 7, 2011): 745–752.
Author's final manuscript