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dc.contributor.authorCha, Jae Won
dc.contributor.authorSingh, Vijay Raj
dc.contributor.authorKim, Ki Hean
dc.contributor.authorSubramanian, Jaichandar
dc.contributor.authorPeng, Qiwen
dc.contributor.authorYu, Hanry
dc.contributor.authorNedivi, Elly
dc.contributor.authorSo, Peter T. C.
dc.date.accessioned2014-07-08T16:02:25Z
dc.date.available2014-07-08T16:02:25Z
dc.date.issued2014-06
dc.date.submitted2014-02
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/88188
dc.description.abstractMultifocal multiphoton microscopy (MMM) achieves fast imaging by simultaneously scanning multiple foci across different regions of specimen. The use of imaging detectors in MMM, such as CCD or CMOS, results in degradation of image signal-to-noise-ratio (SNR) due to the scattering of emitted photons. SNR can be partly recovered using multianode photomultiplier tubes (MAPMT). In this design, however, emission photons scattered to neighbor anodes are encoded by the foci scan location resulting in ghost images. The crosstalk between different anodes is currently measured a priori, which is cumbersome as it depends specimen properties. Here, we present the photon reassignment method for MMM, established based on the maximum likelihood (ML) estimation, for quantification of crosstalk between the anodes of MAPMT without a priori measurement. The method provides the reassignment of the photons generated by the ghost images to the original spatial location thus increases the SNR of the final reconstructed image.en_US
dc.description.sponsorshipRO1 EY017656en_US
dc.description.sponsorshipSingapore-MIT Alliance for Research and Technologyen_US
dc.description.sponsorshipNIH P41EB015871en_US
dc.description.sponsorship5 R01 NS051320en_US
dc.description.sponsorship4R44EB012415en_US
dc.description.sponsorshipNSF CBET-0939511en_US
dc.description.sponsorshipMIT Skoltech Initiativeen_US
dc.description.sponsorshipDavid H. Koch Institute for Integrative Cancer Research at MITen_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep05153en_US
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceNature Publishing Groupen_US
dc.titleReassignment of Scattered Emission Photons in Multifocal Multiphoton Microscopyen_US
dc.typeArticleen_US
dc.identifier.citationCha, Jae Won, Vijay Raj Singh, Ki Hean Kim, Jaichandar Subramanian, Qiwen Peng, Hanry Yu, Elly Nedivi, and Peter T. C. So. “Reassignment of Scattered Emission Photons in Multifocal Multiphoton Microscopy.” Sci. Rep. 4 (June 5, 2014).en_US
dc.contributor.departmentMassachusetts Institute of Technology. Center for Biomedical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.departmentPicower Institute for Learning and Memoryen_US
dc.contributor.mitauthorCha, Jae Wonen_US
dc.contributor.mitauthorSo, Peter T. C.en_US
dc.contributor.mitauthorSubramanian, Jaichandaren_US
dc.contributor.mitauthorNedivi, Ellyen_US
dc.contributor.mitauthorYu, Hanryen_US
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsCha, Jae Won; Singh, Vijay Raj; Kim, Ki Hean; Subramanian, Jaichandar; Peng, Qiwen; Yu, Hanry; Nedivi, Elly; So, Peter T. C.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-0339-3685
dc.identifier.orcidhttps://orcid.org/0000-0002-1710-0767
dc.identifier.orcidhttps://orcid.org/0000-0003-4698-6488
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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