dc.contributor.author | Cha, Jae Won | |
dc.contributor.author | Singh, Vijay Raj | |
dc.contributor.author | Kim, Ki Hean | |
dc.contributor.author | Subramanian, Jaichandar | |
dc.contributor.author | Peng, Qiwen | |
dc.contributor.author | Yu, Hanry | |
dc.contributor.author | Nedivi, Elly | |
dc.contributor.author | So, Peter T. C. | |
dc.date.accessioned | 2014-07-08T16:02:25Z | |
dc.date.available | 2014-07-08T16:02:25Z | |
dc.date.issued | 2014-06 | |
dc.date.submitted | 2014-02 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/88188 | |
dc.description.abstract | Multifocal multiphoton microscopy (MMM) achieves fast imaging by simultaneously scanning multiple foci across different regions of specimen. The use of imaging detectors in MMM, such as CCD or CMOS, results in degradation of image signal-to-noise-ratio (SNR) due to the scattering of emitted photons. SNR can be partly recovered using multianode photomultiplier tubes (MAPMT). In this design, however, emission photons scattered to neighbor anodes are encoded by the foci scan location resulting in ghost images. The crosstalk between different anodes is currently measured a priori, which is cumbersome as it depends specimen properties. Here, we present the photon reassignment method for MMM, established based on the maximum likelihood (ML) estimation, for quantification of crosstalk between the anodes of MAPMT without a priori measurement. The method provides the reassignment of the photons generated by the ghost images to the original spatial location thus increases the SNR of the final reconstructed image. | en_US |
dc.description.sponsorship | RO1 EY017656 | en_US |
dc.description.sponsorship | Singapore-MIT Alliance for Research and Technology | en_US |
dc.description.sponsorship | NIH P41EB015871 | en_US |
dc.description.sponsorship | 5 R01 NS051320 | en_US |
dc.description.sponsorship | 4R44EB012415 | en_US |
dc.description.sponsorship | NSF CBET-0939511 | en_US |
dc.description.sponsorship | MIT Skoltech Initiative | en_US |
dc.description.sponsorship | David H. Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.language.iso | en_US | |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/srep05153 | en_US |
dc.rights | Creative Commons Attribution-NonCommercial-ShareAlike 3.0 | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
dc.source | Nature Publishing Group | en_US |
dc.title | Reassignment of Scattered Emission Photons in Multifocal Multiphoton Microscopy | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Cha, Jae Won, Vijay Raj Singh, Ki Hean Kim, Jaichandar Subramanian, Qiwen Peng, Hanry Yu, Elly Nedivi, and Peter T. C. So. “Reassignment of Scattered Emission Photons in Multifocal Multiphoton Microscopy.” Sci. Rep. 4 (June 5, 2014). | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Center for Biomedical Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Mechanical Engineering | en_US |
dc.contributor.department | Picower Institute for Learning and Memory | en_US |
dc.contributor.mitauthor | Cha, Jae Won | en_US |
dc.contributor.mitauthor | So, Peter T. C. | en_US |
dc.contributor.mitauthor | Subramanian, Jaichandar | en_US |
dc.contributor.mitauthor | Nedivi, Elly | en_US |
dc.contributor.mitauthor | Yu, Hanry | en_US |
dc.relation.journal | Scientific Reports | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Cha, Jae Won; Singh, Vijay Raj; Kim, Ki Hean; Subramanian, Jaichandar; Peng, Qiwen; Yu, Hanry; Nedivi, Elly; So, Peter T. C. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-0339-3685 | |
dc.identifier.orcid | https://orcid.org/0000-0002-1710-0767 | |
dc.identifier.orcid | https://orcid.org/0000-0003-4698-6488 | |
mit.license | PUBLISHER_CC | en_US |
mit.metadata.status | Complete | |