Increased Levels of Inosine in a Mouse Model of Inflammation
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Prestwich, Erin; Mangerich, Aswin; Pang, Bo; McFaline, Jose Luis; Lonkar, Pallavi; Sullivan, Matthew R.; Trudel, Laura J.; Taghizedeh, Koli; Dedon, Peter C.; ... Show more Show less
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One possible mechanism linking inflammation with cancer involves the generation of reactive oxygen, nitrogen, and halogen species by activated macrophages and neutrophils infiltrating sites of infection or tissue damage, with these chemical mediators causing damage that ultimately leads to cell death and mutation. To determine the most biologically deleterious chemistries of inflammation, we previously assessed products across the spectrum of DNA damage arising in inflamed tissues in the SJL mouse model nitric oxide overproduction (Pang et al. (2007) Carcinogenesis28, 1807−1813). Among the anticipated DNA damage chemistries, we observed significant changes only in lipid peroxidation-derived etheno adducts. We have now developed an isotope-dilution, liquid chromatography-coupled, tandem quadrupole mass spectrometric method to quantify representative species across the spectrum of RNA damage products predicted to arise at sites of inflammation, including nucleobase deamination (xanthosine and inosine), oxidation (8-oxoguanosine), and alkylation (1,N6-ethenoadenosine). Application of the method to the liver, spleen, and kidney from the SJL mouse model revealed generally higher levels of oxidative background RNA damage than was observed in DNA in control mice. However, compared to control mice, RcsX treatment to induce nitric oxide overproduction resulted in significant increases only in inosine and only in the spleen. Further, the nitric oxide synthase inhibitor, N-methylarginine, did not significantly affect the levels of inosine in control and RcsX-treated mice. The differences between DNA and RNA damage in the same animal model of inflammation point to possible influences from DNA repair, RcsX-induced alterations in adenosine deaminase activity, and differential accessibility of DNA and RNA to reactive oxygen and nitrogen species as determinants of nucleic acid damage during inflammation.
Date issued
2013-04Department
Massachusetts Institute of Technology. Center for Environmental Health Sciences; Massachusetts Institute of Technology. Department of Biological EngineeringJournal
Chemical Research in Toxicology
Publisher
American Chemical Society
Citation
Prestwich, Erin G., Aswin Mangerich, Bo Pang, Jose L. McFaline, Pallavi Lonkar, Matthew R. Sullivan, Laura J. Trudel, Koli Taghizedeh, and Peter C. Dedon. “Increased Levels of Inosine in a Mouse Model of Inflammation.” Chemical Research in Toxicology 26, no. 4 (April 15, 2013): 538–546.
Version: Author's final manuscript
ISSN
0893-228X
1520-5010