Direct observation of mammalian cell growth and size regulation
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We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro–B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.
Date issued
2012-08Department
Massachusetts Institute of Technology. Computational and Systems Biology Program; Massachusetts Institute of Technology. Department of Mechanical Engineering; Koch Institute for Integrative Cancer Research at MITJournal
Nature Methods
Publisher
Nature Publishing Group
Citation
Son, Sungmin, Amit Tzur, Yaochung Weng, Paul Jorgensen, Jisoo Kim, Marc W Kirschner, and Scott R Manalis. “Direct Observation of Mammalian Cell Growth and Size Regulation.” Nature Methods 9, no. 9 (August 5, 2012): 910–912.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105