Quantifying Particle Coatings Using High-Precision Mass Measurements
Author(s)Knudsen, Scott Michael; von Muhlen, Marcio G.; Manalis, Scott R.
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We present a general method to quantify coatings on microparticle surfaces based on the additional mass. Particle buoyant mass is determined in a solution with a density that is nearly equivalent to that of the core particle, reducing the magnitude and uncertainty of the measurement. Under these conditions, added material with a different density than that of the core is a larger fraction of the total buoyant mass of the coated particle. This method can resolve a buoyant mass difference between uncoated and coated particles of 1 fg. For the protein layer on the 3 μm polystyrene spheres measured herein, this is equivalent to 1/10th of a full layer.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering
American Chemical Society
Knudsen, Scott M., Marcio G. von Muhlen, and Scott R. Manalis. “Quantifying Particle Coatings Using High-Precision Mass Measurements.” Analytical Chemistry 84, no. 3 (February 7, 2012): 1240–1242.
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