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dc.contributor.authorAich, Udayanath
dc.contributor.authorBeckley, Nia
dc.contributor.authorShriver, Zachary H.
dc.contributor.authorRaman, Rahul
dc.contributor.authorViswanathan, Karthik
dc.contributor.authorHobbie, Sven N.
dc.contributor.authorSasisekharan, Ram
dc.date.accessioned2014-09-03T17:58:16Z
dc.date.available2014-09-03T17:58:16Z
dc.date.issued2011-05
dc.date.submitted2011-02
dc.identifier.issn1742464X
dc.identifier.urihttp://hdl.handle.net/1721.1/89155
dc.description.abstractAgglutination of red blood cells (RBCs), including chicken RBCs (cRBCs), has been used extensively to estimate viral titer, to screen glycan-receptor binding preference, and to assess the protective response of vaccines. Although this assay enjoys widespread use, some virus strains do not agglutinate RBCs. To address these underlying issues and to increase the usefulness of cRBCs as tools for studying viruses, such as influenza, we analyzed the cell surface N-glycans of cRBCs. On the basis of the results obtained from complementary analytical strategies, including MS, 1D and 2D-NMR spectroscopy, exoglycosidase digestions, and HPLC profiling, we report the major glycan structures present on cRBCs. By comparing the glycan structures of cBRCs with those of representative human upper respiratory cells, we offer a possible explanation for the fact that certain influenza strains do not agglutinate cRBCs, using specific human-adapted influenza hemagglutinins as examples. Finally, recent understanding of the role of various glycan structures in high affinity binding to influenza hemagglutinins provides context to our findings. These results illustrate that the field of glycomics can provide important information with respect to the experimental systems used to characterize, detect and study viruses.en_US
dc.description.sponsorshipSingapore-MIT Alliance for Research and Technologyen_US
dc.language.isoen_US
dc.publisherJohn Wiley & Sons, Incen_US
dc.relation.isversionofhttp://dx.doi.org/10.1111/j.1742-4658.2011.08096.xen_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleGlycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assayen_US
dc.typeArticleen_US
dc.identifier.citationAich, Udayanath, Nia Beckley, Zachary Shriver, Rahul Raman, Karthik Viswanathan, Sven Hobbie, and Ram Sasisekharan. “Glycomics-Based Analysis of Chicken Red Blood Cells Provides Insight into the Selectivity of the Viral Agglutination Assay.” FEBS Journal 278, no. 10 (April 20, 2011): 1699–1712.en_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorShriver, Zachary H.en_US
dc.contributor.mitauthorBeckley, Niaen_US
dc.contributor.mitauthorAich, Udayanathen_US
dc.contributor.mitauthorRaman, Rahulen_US
dc.contributor.mitauthorViswanathan, Karthiken_US
dc.contributor.mitauthorSasisekharan, Ramen_US
dc.relation.journalFEBS Journalen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsAich, Udayanath; Beckley, Nia; Shriver, Zachary; Raman, Rahul; Viswanathan, Karthik; Hobbie, Sven; Sasisekharan, Ramen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-1288-9965
dc.identifier.orcidhttps://orcid.org/0000-0001-9344-0205
dc.identifier.orcidhttps://orcid.org/0000-0002-2085-7840
mit.licenseOPEN_ACCESS_POLICYen_US


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